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DLK1 Antibody (PA5-97663) in ICC/IF
Immunofluorescent analysis of DLK1 in HepG2 cells using a DLK1 polyclonal antibody (Product # PA5-97663) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-97663
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Protein delta homolog 1 protein (24-303AA)
Conjugate
Form
Liquid
Concentration
4 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2812278
Target Information
The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: delta-like 1 homolog; DLK-1; fetal antigen 1; pG2; Preadipocyte factor 1; preadipocyte factor-1; PREF; Pref-1; Protein delta homolog 1; secredeltin
Gene Aliases: Delta1; DLK; DLK-1; DLK1; FA1; pG2; Pref-1; PREF1; ZOG
UniProt ID: (Human) P80370, (Rat) O70534
Entrez Gene ID: (Human) 8788, (Rat) 114587
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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