Invitrogen

DNA-PK Monoclonal Antibody (18-2)

9 Published Figures

46 References

View all (53) DNA-PK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (9)

DNA-PK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

DNA-PK Antibody (MA5-13238) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with DNAKcs antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes.

DNA-PK Antibody in Immunoprecipitation (IP)

DNA-PK Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA5-13238

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg/mL

View 5 publications 5 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

18-2

Immunogen

Human DNA-dependent Protein Kinase purified from HeLa cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10988612

Product Specific Information

MA5-13238 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13238 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA-PKcs and ATM modulate mitochondrial ADP-ATP exchange as an oxidative stress checkpoint mechanism.

The EMBO journal

Chen WM,Chiang JC,Shang Z,Palchik G,Newman C,Zhang Y,Davis AJ,Lee H,Chen BP

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Western Blot

Wed Mar 15 00:00:00 EDT 2023

DNA-PKcs and ATM modulate mitochondrial ADP-ATP exchange as an oxidative stress checkpoint mechanism.

The EMBO journal

Chen WM,Chiang JC,Shang Z,Palchik G,Newman C,Zhang Y,Davis AJ,Lee H,Chen BP

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Western Blot

Wed Mar 15 00:00:00 EDT 2023

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

MA5-13238 was used in Western Blot to find that retinoic acid-inducible gene I (RIG-I), a key cytosolic RNA sensor that recognizes RNA virus and initiates the MAVS-IRF3-type I IFN signaling cascade, is recruited to double-stranded breaks (DSBs) and suppresses non-homologous end joining (NHEJ).

Mon Apr 12 00:00:00 EDT 2021

HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.

Cell death & disease

Guo Z,Wang S,Xie Y,Han Y,Hu S,Guan H,Xie D,Bai C,Liu X,Gu Y,Zhou PK,Ma T

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Western Blot

Tue May 26 00:00:00 EDT 2020

HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.

Cell death & disease

Guo Z,Wang S,Xie Y,Han Y,Hu S,Guan H,Xie D,Bai C,Liu X,Gu Y,Zhou PK,Ma T

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Western Blot

Tue May 26 00:00:00 EDT 2020

The Ig superfamily protein PTGFRN coordinates survival signaling in glioblastoma multiforme.

Cancer letters

Aguila B,Morris AB,Spina R,Bar E,Schraner J,Vinkler R,Sohn JW,Welford SM

MA5-13238 was used in Western Blotting to show the immunoglobulin superfamily protein PTGFRN influences aggressiveness in glioblastoma multiforme.

Thu Oct 10 00:00:00 EDT 2019

DNA‑PKcs PARylation regulates DNA‑PK kinase activity in the DNA damage response.

Molecular medicine reports

Han Y,Jin F,Xie Y,Liu Y,Hu S,Liu XD,Guan H,Gu Y,Ma T,Zhou PK

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Immunoprecipitation

Tue Oct 01 00:00:00 EDT 2019

DNA‑PKcs PARylation regulates DNA‑PK kinase activity in the DNA damage response.

Molecular medicine reports

Han Y,Jin F,Xie Y,Liu Y,Hu S,Liu XD,Guan H,Gu Y,Ma T,Zhou PK

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Immunoprecipitation

Tue Oct 01 00:00:00 EDT 2019

DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.

Science (New York, N.Y.)

Ochi T,Blackford AN,Coates J,Jhujh S,Mehmood S,Tamura N,Travers J,Wu Q,Draviam VM,Robinson CV,Blundell TL,Jackson SP

MA5-13238 was used in western blot to determine how PAXX interacts with Ku to promote DNA double-strand break repair

Fri Jan 09 00:00:00 EST 2015

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

MA5-13238 was used in immunoprecipitation to study the role of autophagy factors in cancer progression and treatment response

Mon Aug 03 00:00:00 EDT 2015

A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair.

The Journal of cell biology

Britton S,Coates J,Jackson SP

MA5-13238 was used in western blot to develop a new method to visualize DNA double-strand break complexes

Mon Aug 05 00:00:00 EDT 2013

p21 promotes error-free replication-coupled DNA double-strand break repair.

Nucleic acids research

Mauro M,Rego MA,Boisvert RA,Esashi F,Cavallo F,Jasin M,Howlett NG

MA5-13238 was used in western blot to study the role of p21 in error-free replication-coupled DNA double-strand break repair.

Sat Sep 01 00:00:00 EDT 2012

Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.

International journal of radiation oncology, biology, physics

Kang KB,Zhu C,Wong YL,Gao Q,Ty A,Wong MC

MA5-13238 was used in western blot to study the molecular mechanism by which gefitinib radiosensitizes brain tumor stem cells

Tue May 01 00:00:00 EDT 2012

DNA ends alter the molecular composition and localization of Ku multicomponent complexes.

Molecular & cellular proteomics : MCP

Adelmant G,Calkins AS,Garg BK,Card JD,Askenazi M,Miron A,Sobhian B,Zhang Y,Nakatani Y,Silver PA,Iglehart JD,Marto JA,Lazaro JB

MA5-13238 was used in Western Blotting to define the composition of Ku multicomponent complexes, and demonstrate that they are dramatically altered in response to UV radiation.

Wed Aug 01 00:00:00 EDT 2012

Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.

Nucleic acids research

Cheng Q,Barboule N,Frit P,Gomez D,Bombarde O,Couderc B,Ren GS,Salles B,Calsou P

MA5-13238 was used in western blot to study the role of PARP1 and MRN proteins in the repair of DNA double strand breaks

Thu Dec 01 00:00:00 EST 2011

The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.

Journal of cellular physiology

Yu X,Wang H,Wang P,Chen BP,Wang Y

MA5-13238 was used in western blot to study low-dose radiation induction of cell survival and the mechanistic role of Ku-dependent non-homologous end joining

Tue Feb 01 00:00:00 EST 2011

Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin.

Molecular cancer

Sand-Dejmek J,Adelmant G,Sobhian B,Calkins AS,Marto J,Iglehart DJ,Lazaro JB

MA5-13238 was used in Western Blotting to elucidate the mechanism underlying the effects of DNA-dependent protein kinase on cisplatin sensitivity.

Thu Jun 16 00:00:00 EDT 2011

Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.

Genes & development

Takai H,Xie Y,de Lange T,Pavletich NP

MA5-13238 was used in western blot to study the role of Tel2 in the Hsp90-dependent maturation of mTOR and ATR complexes

Wed Sep 15 00:00:00 EDT 2010

Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.

Cell cycle (Georgetown, Tex.)

Velkova A,Carvalho MA,Johnson JO,Tavtigian SV,Monteiro AN

MA5-13238 was used in western blot to study the interaction between the BRCA protein and filamin A and its role in efficient DNA repair

Thu Apr 01 00:00:00 EDT 2010

Defective Artemis causes mild telomere dysfunction.

Genome integrity

Yasaei H,Slijepcevic P

MA5-13238 was used in western blot to study the role of defective Artemis in telomere dysfunction

Wed May 26 00:00:00 EDT 2010

The effect of DNA-dependent protein kinase on adeno-associated virus replication.

PloS one

Choi YK,Nash K,Byrne BJ,Muzyczka N,Song S

MA5-13238 was used in blocking or activating experiment to study the role of DNA-dependent protein kinase in adeno-associated virus replication

Mon Dec 20 00:00:00 EST 2010

ARTEMIS nuclease facilitates apoptotic chromatin cleavage.

Cancer research

Britton S,Frit P,Biard D,Salles B,Calsou P

MA5-13238 was used in western blot to study the role of ARTEMIS nuclease in apoptotic chromatin cleavage

Thu Oct 15 00:00:00 EDT 2009

Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.

Journal of virology

Daya S,Cortez N,Berns KI

MA5-13238 was used in immunoprecipitation to study the mechanism of adeno-associated virus site-specific integration

Sun Nov 01 00:00:00 EDT 2009

DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.

Biochemical and biophysical research communications

Choul-li S,Drobecq H,Aumercier M

MA5-13238 was used in western blot to study the interaction of DNA-PK with the Ets-1 transcription factor

Fri Dec 18 00:00:00 EST 2009

Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.

Rheumatology (Oxford, England)

Schild-Poulter C,Su A,Shih A,Kelly OP,Fritzler MJ,Goldstein R,Haché RJ

MA5-13238 was used in ChIP assay to study autoantibodies to Ku and DNA repair proteins in connective tissue diseases

Fri Feb 01 00:00:00 EST 2008

Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis.

PloS one

Meulle A,Salles B,Daviaud D,Valet P,Muller C

Published figure using DNA-PK monoclonal antibody (Product # MA5-13238) in Western Blot

Wed Nov 12 00:00:00 EST 2008

Analytical biochemistry of DNA--protein assemblies from crude cell extracts.

Nucleic acids research

Hégarat N,Cardoso GM,Rusconi F,Francois JC,Praseuth D

MA5-13238 was used in western blot to develop an efficient method for the purification of DNA-protein complexes from crude extracts

Wed Aug 15 00:00:00 EDT 2007

Adenovirus-mediated expression of a dominant negative Ku70 fragment radiosensitizes human tumor cells under aerobic and hypoxic conditions.

Cancer research

He F,Li L,Kim D,Wen B,Deng X,Gutin PH,Ling CC,Li GC

MA5-13238 was used in Western Blotting to examine the use of a radio-gene therapy paradigm to sensitise cells to ionising radiation.

Mon Jan 15 00:00:00 EST 2007

The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Kiefer K,Oshinsky J,Kim J,Nakajima PB,Bosma GC,Bosma MJ

MA5-13238 was used in western blot to study the mechanism for Ig class-switch recombination

Tue Feb 20 00:00:00 EST 2007

Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.

The Journal of biological chemistry

Wu PY,Frit P,Malivert L,Revy P,Biard D,Salles B,Calsou P

MA5-13238 was used in western blot to study the roles of Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends

Fri Nov 02 00:00:00 EDT 2007

DNA end joining activity is reduced in Alzheimer's disease.

Neurobiology of aging

Shackelford DA

MA5-13238 was used in western blot to study the role of DNA-dependent protein kinase in reduced DNA end joining activity in Alzheimer's disease

Sat Apr 01 00:00:00 EST 2006

The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.

The Journal of experimental medicine

Poinsignon C,Moshous D,Callebaut I,de Chasseval R,Villey I,de Villartay JP

MA5-13238 was used in western blot to study the role of the metallo-beta-lactamase/beta-CASP domain of Artemis as the catalytic core for V(D)J recombination

Mon Feb 02 00:00:00 EST 2004

DNA-dependent PK inhibits adeno-associated virus DNA integration.

Proceedings of the National Academy of Sciences of the United States of America

Song S,Lu Y,Choi YK,Han Y,Tang Q,Zhao G,Berns KI,Flotte TR

MA5-13238 was used in western blot to study the inhibitory effect of DNA-PK on adeno-associated virus integration in mouse liver in vitro and in vivo

Tue Feb 17 00:00:00 EST 2004

Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.

Oncogene

Loong SL,Korzh S,Price A

MA5-13238 was used in western blot to study the reduced DNA-PK activity of two immortalized lymphoblastoid cell lines from adult cancer patients suffering late radionecrosis and the significance for radionecrosis susceptibility

Thu Jul 15 00:00:00 EDT 2004

Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.

Journal of immunology (Baltimore, Md. : 1950)

Repasky JA,Corbett E,Boboila C,Schatz DG

MA5-13238 was used in immunoprecipitation to study the role of terminal deoxynucleotidyltransferase in V(D)J recombination

Sat May 01 00:00:00 EDT 2004

Backup pathways of NHEJ are suppressed by DNA-PK.

Journal of cellular biochemistry

Perrault R,Wang H,Wang M,Rosidi B,Iliakis G

MA5-13238 was used in western blot to study the suppression of alternative, backup pathways of non-homolgous end joining by DNA-PK

Thu Jul 01 00:00:00 EDT 2004

Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.

The Journal of biological chemistry

Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P

MA5-13238 was used in western blot to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity

Fri Jul 09 00:00:00 EDT 2004

Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.

Nucleic acids research

Onclercq-Delic R,Calsou P,Delteil C,Salles B,Papadopoulo D,Amor-Guéret M

MA5-13238 was used in western blot to study the potential anti-recombinogenic role of BLM DNA helicase in double-strand break processing

Sat Nov 01 00:00:00 EST 2003

Biochemical evidence for Ku-independent backup pathways of NHEJ.

Nucleic acids research

Wang H,Perrault AR,Takeda Y,Qin W,Wang H,Iliakis G

MA5-13238 was used in western blot to study alternative backup pathways of non-homologous end joining that are independent of Ku

Mon Sep 15 00:00:00 EDT 2003

Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.

Journal of molecular biology

Calsou P,Delteil C,Frit P,Drouet J,Salles B

MA5-13238 was used in western blot to investigate the relationship and assembly of specific proteins during DNA repair process

Fri Feb 07 00:00:00 EST 2003

Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.

American journal of physiology. Cell physiology

Lebrasseur NK,Coté GM,Miller TA,Fielding RA,Sawyer DB

MA5-13238 was used in western blot to study the regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle

Thu May 01 00:00:00 EDT 2003

Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.

Head & neck

Friesland S,Kanter-Lewensohn L,Tell R,Munck-Wikland E,Lewensohn R,Nilsson A

MA5-13238 was used in immunohistochemistry to study the correlation between Ku86 expression and favorable outcome in tonsillar carcinoma treated with radiotherapy

Tue Apr 01 00:00:00 EST 2003

Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.

The Journal of biological chemistry

Udayakumar D,Bladen CL,Hudson FZ,Dynan WS

MA5-13238 was used in western blot to study two biochemically distinct pathways of non-homologous end joining that are differentially regulated by DNA-PK

Fri Oct 24 00:00:00 EDT 2003

The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.

Molecular and cellular biology

Jhappan C,Yusufzai TM,Anderson S,Anver MR,Merlino G

MA5-13238 was used in western blot to study whether DNA-dependent protein kinase is involved in the p53-mediated response to DNA damage

Thu Jun 01 00:00:00 EDT 2000

Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.

The Journal of biological chemistry

Chen L,Trujillo K,Sung P,Tomkinson AE

MA5-13238 was used in western blot to study the interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase

Fri Aug 25 00:00:00 EDT 2000

Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.

Molecular and cellular biology

Giffin W,Gong W,Schild-Poulter C,Haché RJ

MA5-13238 was used in EMSA to study the mechanism by which Ku antigen-DNA conformation modulates the repression of MMTV transcription

Tue Jun 01 00:00:00 EDT 1999

A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.

Immunity

Gao Y,Chaudhuri J,Zhu C,Davidson L,Weaver DT,Alt FW

MA5-13238 was used in western blot to study the DNA-PK-independent functions of Ku70 and Ku80 in V(D)J recombination

Tue Sep 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

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Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

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UniProt ID: (Human) P78527

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Entrez Gene ID: (Human) 5591, (Rat) 360748

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Function(s)

double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding molecular_function DNA binding kinase activity non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation telomere maintenance double-strand break repair via homologous recombination immunoglobulin production protein phosphorylation response to ionizing radiation phosphorylation lymphocyte differentiation V(D)J recombination

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DNA-PK Monoclonal Antibody (18-2)

DNA-PK Antibody (MA5-13238) in IHC (P)

Product Details

MA5-13238

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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