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Product Details
MA5-42568
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2-B3
Immunogen
Recombinant protein within human dna-pkcs/prkdc aa 3900-4128
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911709
Product Specific Information
Positive Control: HepG2 Hela
Subcellular Location: Nucleus
Target Information
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; severe combined immunodeficiency
Gene Aliases: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7
UniProt ID: (Human) P78527, (Mouse) P97313
Entrez Gene ID: (Human) 5591, (Mouse) 19090
double-stranded DNA binding
protein kinase activity
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
transcription factor binding
enzyme binding
poly(A) RNA binding
nucleotide binding
DNA binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
non-receptor serine/threonine protein kinase
protein modifying enzyme
somitogenesis
negative regulation of protein phosphorylation
B cell lineage commitment
pro-B cell differentiation
T cell lineage commitment
negative regulation of immunoglobulin production
positive regulation of immune system process
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular protein modification process
brain development
heart development
cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
response to activity
telomere capping
peptidyl-serine phosphorylation
protein destabilization
positive regulation of type I interferon production
cellular response to insulin stimulus
T cell differentiation in thymus
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
ectopic germ cell programmed cell death
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
rhythmic process
spleen development
thymus development
positive regulation of developmental growth
regulation of smooth muscle cell proliferation
signal transduction involved in mitotic G1 DNA damage checkpoint
double-strand break repair via alternative nonhomologous end joining
negative regulation of cellular senescence
negative regulation of response to gamma radiation
telomere maintenance
double-strand break repair via homologous recombination
immunoglobulin production
DNA repair
protein phosphorylation
cellular response to DNA damage stimulus
response to ionizing radiation
phosphorylation
lymphocyte differentiation
V(D)J recombination
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