Invitrogen

DNM1L Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

12 Published Figures

12 References

View all (56) DNM1L antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

DNM1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 20

DNM1L Antibody (PA1-16987) in IHC (P)

Immunohistochemical analysis of DNM1L in renal tubular epithelium and visceral epithelial cells of the glomerulus. Samples were incubated in DNM1L polyclonal antibody (Product # PA1-16987). Human kidney cortex, 40X magnification.

Product Image Gallery

DNM1L Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DNM1L siRNA and reduction of signal was observed in Western Blot using DNM1L Polyclonal Antibody (Product # PA1-16987). {KD}

Product Details

PA1-16987

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

Immunocytochemistry (ICC/IF)

1:500

Flow Cytometry (Flow)

1:1,000

Immunoprecipitation (IP)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 500-600 of human DRP1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2093523

Product Specific Information

This antibody is predicted to react with bovine based on 100% sequence homology.

Suggested positive control: antigen standard for DNM1L (transient overexpression lysate), human kidney protein.

Target Information

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDDO-Me Abrogates Aberrant Mitochondrial Elongation in Clasmatodendritic Degeneration by Regulating NF-κB-PDI-Mediated S-Nitrosylation of DRP1.

International journal of molecular sciences

Lee DS,Kim TH,Park H,Kim JE

Published figure using DNM1L polyclonal antibody (Product # PA1-16987) in Western Blot

Mon Mar 20 00:00:00 EDT 2023

EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus.

Antioxidants (Basel, Switzerland)

Kim JE,Kim TH,Kang TC

PA1-16987 was used in Immunohistochemistry and Western Blotting to suggest that EGCG may protect CA1 neurons from SE insults through GPx1-ERK1/2-DRP1 and GPx1-NF-κB signaling pathways, respectively.

Thu Apr 20 00:00:00 EDT 2023

EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus.

Antioxidants (Basel, Switzerland)

Kim JE,Kim TH,Kang TC

PA1-16987 was used in Immunohistochemistry and Western Blotting to suggest that EGCG may protect CA1 neurons from SE insults through GPx1-ERK1/2-DRP1 and GPx1-NF-κB signaling pathways, respectively.

Thu Apr 20 00:00:00 EDT 2023

EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus.

Antioxidants (Basel, Switzerland)

Kim JE,Kim TH,Kang TC

PA1-16987 was used in Immunohistochemistry and Western Blotting to suggest that EGCG may protect CA1 neurons from SE insults through GPx1-ERK1/2-DRP1 and GPx1-NF-κB signaling pathways, respectively.

Thu Apr 20 00:00:00 EDT 2023

CDDO-Me Attenuates Clasmatodendrosis in CA1 Astrocyte by Inhibiting HSP25-AKT Mediated DRP1-S637 Phosphorylation in Chronic Epilepsy Rats.

International journal of molecular sciences

Lee DS,Kim TH,Park H,Kim JE

Published figure using DNM1L polyclonal antibody (Product # PA1-16987) in Western Blot

Wed Apr 20 00:00:00 EDT 2022

A new model for fatty acid hydroxylase-associated neurodegeneration reveals mitochondrial and autophagy abnormalities.

Frontiers in cell and developmental biology

Mandik F,Kanana Y,Rody J,Misera S,Wilken B,Laabs von Holt BH,Klein C,Vos M

Published figure using DNM1L polyclonal antibody (Product # PA1-16987) in Western Blot

Wed Jan 11 00:00:00 EST 2023

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.

Current biology : CB

Hotta T,McAlear TS,Yue Y,Higaki T,Haynes SE,Nesvizhskii AI,Sept D,Verhey KJ,Bechstedt S,Ohi R

PA1-16987 was used in Western Blot to increase microtubule stability.

Mon Sep 26 00:00:00 EDT 2022

β-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle.

Nutrients

Monsalves-Alvarez M,Morales PE,Castro-Sepulveda M,Sepulveda C,Rodriguez JM,Chiong M,Eisner V,Lavandero S,Troncoso R

PA1-16987 was used in Western Blotting to suggest that short, but not long-term, β-hydroxybutyrate supplementation increases exercise capacity, associated with modifications in mitochondrial morphology and function in mouse skeletal muscle.

Mon Jun 29 00:00:00 EDT 2020

β-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle.

Nutrients

Monsalves-Alvarez M,Morales PE,Castro-Sepulveda M,Sepulveda C,Rodriguez JM,Chiong M,Eisner V,Lavandero S,Troncoso R

Mon Jun 29 00:00:00 EDT 2020

CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.

Cells

Kim JE,Park H,Choi SH,Kong MJ,Kang TC

PA1-16987 was used in Western Blotting to determine the mechanism of neuronal death following status epilepticus.

Mon Aug 05 00:00:00 EDT 2019

PDI-mediated S-nitrosylation of DRP1 facilitates DRP1-S616 phosphorylation and mitochondrial fission in CA1 neurons.

Cell death & disease

Lee DS,Kim JE

Published figure using DNM1L polyclonal antibody (Product # PA1-16987) in Western Blot

Wed Aug 29 00:00:00 EDT 2018

Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death.

Frontiers in cellular neuroscience

Hyun HW,Ko AR,Kang TC

PA1-16987 was used in western blot to facilitate LIM kinase 2-mediated programmed necrotic neuronal death by mitochondrial translocation of high mobility group box 1

Thu May 05 00:00:00 EDT 2016

Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.

Molecular brain

Ko AR,Hyun HW,Min SJ,Kim JE,Kang TC

PA1-16987 was used in western blot to study the role of endothelin-1 in LIMK2-mediated neuronal necrosis

Tue Oct 06 00:00:00 EDT 2015

Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.

Molecular brain

Ko AR,Hyun HW,Min SJ,Kim JE,Kang TC

PA1-16987 was used in western blot to study the role of endothelin-1 in LIMK2-mediated neuronal necrosis

Tue Oct 06 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1

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Gene Aliases: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python

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UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6

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Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006

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Function(s)

GTPase activity GTPase activator activity protein binding GTP binding lipid binding Rab GTPase binding GTP-dependent protein binding ubiquitin protein ligase binding protein homodimerization activity identical protein binding nucleotide binding hydrolase activity membrane traffic protein

Process(es)

mitochondrial fission release of cytochrome c from mitochondria dynamin polymerization involved in mitochondrial fission endocytosis cellular component disassembly involved in execution phase of apoptosis regulation of mitochondrion organization peroxisome fission regulation of protein oligomerization positive regulation of apoptotic process positive regulation of GTPase activity mitochondrial fragmentation involved in apoptotic process positive regulation of protein secretion protein oligomerization protein homotetramerization heart contraction membrane fusion necroptotic process mitochondrion morphogenesis protein localization to mitochondrion positive regulation of mitochondrial fission mitochondrial membrane fission positive regulation of release of cytochrome c from mitochondria regulation of peroxisome organization regulation of mitophagy regulation of ATP metabolic process positive regulation of intrinsic apoptotic signaling pathway metabolic process programmed cell death

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNM1L Polyclonal Antibody

DNM1L Antibody (PA1-16987) in IHC (P)

Product Details

PA1-16987

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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