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DNMT1 Antibody (PA5-120920) in IP
Immunoprecipitation of DNMT1 in 200 μg extracts of 293T cells. Samples were precipitated with 3 μg DNMT1 Polyclonal antibody (Product # PA5-120920). Western blot was performed from the immunoprecipitate using DNMT1 Polyclonal antibody (Product # PA5-120920) at a dilution of 1:1,000.
Product Details
PA5-120920
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914492
Product Specific Information
Positive test controls include: 293T, SW480. The target is usually found in the following locations: Nucleus.
Immunogen sequence: GDCVSVIPDD SSKPLYLARV TALWEDSSNG QMFHAHWFCA GTDTVLGATS DPLELFLVDE CEDMQLSYIH SKVKVIYKAP SENWAMEGGM DPESLLEGDD GKTYFYQLWY DQDYARFESP PKTQPTEDNK FKFCVSCARL AEMRQKEIPR VLEQLEDLDS RVLYYSATKN GILYRVGDGV YLPPEAFTFN IKLSSPVKRP RKEPVDEDLY PEHYRKYSDY IKGSNLDAPE PYRIGRIKEI FCPKKSNGRP NETDIKIRVN KFYRPENTHK STPASYHADI NLLYWSDEEA VVDFKAVQGR CTVEYGEDLP ECVQVYSMGG PNRFYFLEAY NAKSKSFEDP PNHARSPGNK
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase HsaI; DNA MTase HsaI; Dnmt1; FLJ16293; MCMT; MGC104992
Gene Aliases: ADCADN; AIM; CXXC9; DNMT; DNMT1; HSN1E; m.HsaI; MCMT
UniProt ID: (Human) P26358
Entrez Gene ID: (Human) 1786
DNA binding
double-stranded DNA binding
RNA binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
zinc ion binding
methyl-CpG binding
DNA-methyltransferase activity
protein domain specific binding
estrogen receptor binding
histone deacetylase binding
unmethylated CpG binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
promoter-specific chromatin binding
DNA metabolism protein
DNA methyltransferase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
transcription, DNA-templated
Ras protein signal transduction
brain development
aging
response to heat
response to toxic substance
response to ionizing radiation
maintenance of DNA methylation
DNA methylation on cytosine within a CG sequence
positive regulation of gene expression
response to activity
gene silencing
covalent chromatin modification
neuron differentiation
response to caffeine
response to estradiol
response to lipopolysaccharide
response to vitamin A
response to testosterone
cellular response to platelet-derived growth factor stimulus
response to antidepressant
regulation of cell proliferation
DNA hypermethylation
response to ethanol
negative regulation of gene expression, epigenetic
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
S-adenosylmethionine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
cellular response to amino acid stimulus
cellular response to lead ion
cellular response to transforming growth factor beta stimulus
C-5 methylation of cytosine
positive regulation of methylation-dependent chromatin silencing
cellular response to nerve growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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