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DNMT3A Polyclonal Antibody

DNMT3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNMT3A Antibody (20954-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using (Product # 20954-1-AP) (DNMT3A antibody) at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNMT3A Antibody in Immunoprecipitation (IP)

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Product Details

20954-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DNMT3A Fusion Protein Ag15111 (1-280 aa encoded by BC043617)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Immunogen sequence: MPAMPSSGP GDTSSSAAER EEDRKDGEEQ EEPRGKEERQ EPSTTARKVG RPGRKRKHPP VESGDTPKDP AVISKSPSMA QDSGASELLP NGDLEKRSEP QPEEGSPAGG QKGGAPAEGE GAAETLPEAS RAVENGCCTP KEGRGAPAEA GKEQKETNIE SMKMEGSRGR LRGGLGWESS LRQRPMPRLT FQAGDPYYIS KRKRDEWLAR WKREAEKKAK VIAGMNAVEE NQGPGESQKV EEASPPAVQQ PTDPASPTVA TTPEPVGSDA GDKNATKAGD D (1-280 aa encoded by BC043617)

Target Information

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic control of LncRNA NEAT1 enables cardiac fibroblast pyroptosis and cardiac fibrosis.

European journal of pharmacology

Ding JF,Zhou Y,Xu SS,Shi KH,Sun H,Tu B,Song K,Xuan HY,Sha JM,Zhao JY,Tao H

Thu Jan 05 00:00:00 EST 2023

Decreased expression and DNA hypermethylation of syncytin-1 in human villus tissues with unexplained recurrent spontaneous abortion.

Journal of reproductive immunology

Fu Y,Song Y,Zhang J,Wei LP,Sun XR

Wed Feb 01 00:00:00 EST 2023

Hypermethylation of microRNA-497-3p contributes to progression of thyroid cancer through activation of PAK1/β-catenin.

Cell biology and toxicology

Fan Y,Fan X,Yan H,Liu Z,Wang X,Yuan Q,Xie J,Lu X,Yang Y

Sun Oct 01 00:00:00 EDT 2023

Effects of H19/SAHH/DNMT1 on the oxidative DNA damage related to benzo[a]pyrene exposure.

Environmental science and pollution research international

Fu Y,Li X,Pan B,Niu Y,Zhang B,Zhao X,Nie J,Yang J

Sun Jan 01 00:00:00 EST 2023

Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation.

Nature communications

Li Q,Lu J,Yin X,Chang Y,Wang C,Yan M,Feng L,Cheng Y,Gao Y,Xu B,Zhang Y,Wang Y,Cui G,Xu L,Sun Y,Zeng R,Li Y,Jing N,Xu GL,Wu L,Tang F,Li J

Mon May 22 00:00:00 EDT 2023

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.

Protein & cell

Zhang H,Li Y,Ma Y,Lai C,Yu Q,Shi G,Li J

Tue Feb 01 00:00:00 EST 2022

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

International journal of biological sciences

Miao J,Zhao C,Tang K,Xiong X,Wu F,Xue W,Duan B,Zhang H,Jing X,Li W,Sun Y,Hou N,Huang C

Thu Apr 14 00:00:00 EDT 2022

CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression.

Biomedicines

Jin Z,Ye J,Chen S,Ren Y,Guo W

Wed Nov 23 00:00:00 EST 2022

Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity.

Science advances

Chen X,Zhou W,Song RH,Liu S,Wang S,Chen Y,Gao C,He C,Xiao J,Zhang L,Wang T,Liu P,Duan K,Cheng Z,Zhang C,Zhang J,Sun Y,Jackson F,Lan F,Liu Y,Xu Y,Wong JJ,Wang P,Yang H,Xiong Y,Chen T,Li Y,Ye D

Fri Jan 28 00:00:00 EST 2022

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis.

Psychiatry investigation

Ming Y,Deng Z,Tian X,Jia Y,Ning M,Cheng S

Sat Oct 01 00:00:00 EDT 2022

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis.

Psychiatry investigation

Ming Y,Deng Z,Tian X,Jia Y,Ning M,Cheng S

Sat Oct 01 00:00:00 EDT 2022

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.

International journal of biological macromolecules

Wang R,Yang M,Wu Y,Liu R,Liu M,Li Q,Su X,Xin Y,Huo W,Deng Q,Ba Y,Huang H

Fri Sep 30 00:00:00 EDT 2022

CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug.

Molecular cancer

Liang Y,Cen J,Huang Y,Fang Y,Wang Y,Shu G,Pan Y,Huang K,Dong J,Zhou M,Xu Y,Luo J,Liu M,Zhang J

Mon Dec 19 00:00:00 EST 2022

CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug.

Molecular cancer

Liang Y,Cen J,Huang Y,Fang Y,Wang Y,Shu G,Pan Y,Huang K,Dong J,Zhou M,Xu Y,Luo J,Liu M,Zhang J

Mon Dec 19 00:00:00 EST 2022

Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1β-Induced Osteoarthritis Progression Through Regulating DNMT3A.

Frontiers in genetics

Zhu H,Zhu S,Shang X,Meng X,Jing S,Yu L,Deng Y

Tue Jun 08 00:00:00 EDT 2021

piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer.

Frontiers in cell and developmental biology

Ding X,Li Y,Lü J,Zhao Q,Guo Y,Lu Z,Ma W,Liu P,Pestell RG,Liang C,Yu Z

Tue May 11 00:00:00 EDT 2021

Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.

Integrative cancer therapies

Wang L,Xu L,Wang Y

Mon Oct 11 00:00:00 EDT 2021

Coeloglossum viride var. bracteatum extract attenuates staurosporine induced neurotoxicity by restoring the FGF2-PI3K/Akt signaling axis and Dnmt3.

Heliyon

Cai ZP,Cao C,Guo Z,Yu Y,Zhong SJ,Pan RY,Liang H,Lan R,Qin XY

Thu Jul 01 00:00:00 EDT 2021

DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.

Biological research

Zhu Y,Wang X,Zhou X,Ding L,Liu D,Xu H

Fri Aug 06 00:00:00 EDT 2021

Methylation gene KCNC1 is associated with overall survival in patients with seminoma.

Oncology reports

Chen S,Xiao L,Peng H,Wang Z,Xie J

Sat May 01 00:00:00 EDT 2021

Downregulation of DNMT3a expression by RNAi and its effect on NF-κBs expression of thymic epithelial cells.

Immunology letters

Meng FJ,Guo F,Sun ZN,Wang SJ,Yang CR,Wang CY,Zhang WC,Gao ZY,Ji LL,Feng FK,Guan ZY,Wang GS

Wed Sep 01 00:00:00 EDT 2021

Upregulation of programmed death ligand 1 by liver kinase B1 and its implication in programmed death 1 blockade therapy in non-small cell lung cancer.

Life sciences

Shen X,Zhao Y,Liu G,Zhou HL,Fan J,Zhang L,Li YL,Wang Y,Liang J,Xu ZX

Tue Sep 01 00:00:00 EDT 2020

Radiation-Induced DNMT3B Promotes Radioresistance in Nasopharyngeal Carcinoma through Methylation of p53 and p21.

Molecular therapy oncolytics

Wu C,Guo E,Ming J,Sun W,Nie X,Sun L,Peng S,Luo M,Liu D,Zhang L,Mei Q,Long G,Hu G,Hu G

Fri Jun 26 00:00:00 EDT 2020

Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.

Brain sciences

Sivalingam K,Samikkannu T

Sat Sep 05 00:00:00 EDT 2020

Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.

Epigenetics & chromatin

Buck JM,O'Neill HC,Stitzel JA

Thu Mar 05 00:00:00 EST 2020

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Journal of molecular and cellular cardiology

Zhu X,Du J,Yu J,Guo R,Feng Y,Qiao L,Xu Z,Yang F,Zhong G,Liu F,Cheng F,Chu M,Lin J

Tue Jan 01 00:00:00 EST 2019

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Journal of molecular and cellular cardiology

Zhu X,Du J,Yu J,Guo R,Feng Y,Qiao L,Xu Z,Yang F,Zhong G,Liu F,Cheng F,Chu M,Lin J

Tue Jan 01 00:00:00 EST 2019

miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.

Cell biology international

Xu S,Zhang B,Zhu Y,Huang H,Yang W,Huang H,Zheng HL,Liu X

Wed Mar 01 00:00:00 EST 2017

miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.

Cell biology international

Xu S,Zhang B,Zhu Y,Huang H,Yang W,Huang H,Zheng HL,Liu X

Wed Mar 01 00:00:00 EST 2017

Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.

Research in veterinary science

Wang X,Wang Z,Wang Q,Wang H,Liang H,Liu D

Sun Oct 01 00:00:00 EDT 2017

Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.

Research in veterinary science

Wang X,Wang Z,Wang Q,Wang H,Liang H,Liu D

Sun Oct 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3A; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664

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Gene Aliases: DNMT3A; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS

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UniProt ID: (Human) Q9Y6K1, (Mouse) O88508, (Rat) Q1LZ53

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Entrez Gene ID: (Human) 1788, (Mouse) 13435, (Rat) 444984

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Function(s)

DNA binding chromatin binding DNA (cytosine-5-)-methyltransferase activity protein binding DNA-methyltransferase activity identical protein binding unmethylated CpG binding metal ion binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates methyltransferase activity transferase activity DNA methyltransferase

Process(es)

negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle DNA methylation methylation-dependent chromatin silencing regulation of gene expression by genetic imprinting spermatogenesis aging response to toxic substance response to ionizing radiation response to lead ion DNA methylation on cytosine within a CG sequence positive regulation of cell death neuron differentiation response to estradiol response to vitamin A response to antidepressant response to cocaine DNA methylation involved in embryo development DNA methylation involved in gamete generation hypermethylation of CpG island negative regulation of gene expression, epigenetic S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process cellular response to amino acid stimulus cellular response to ethanol cellular response to hypoxia C-5 methylation of cytosine hepatocyte apoptotic process transcription, DNA-templated regulation of transcription, DNA-templated maintenance of DNA methylation chromatin modification methylation DNA methylation on cytosine response to nutrient levels response to drug response to ethanol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNMT3A Polyclonal Antibody

DNMT3A Antibody (20954-1-AP) in IHC (P)

Product Details

20954-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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