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DNMT3A Antibody (BS-53128R) in ELISA
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Bioss antibody directed against human DNMT3A (Cat. No. bs-53128R), crude serum and Flow Through, in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:500.
Product Details
BS-53128R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; Dnmt3a; W91664
Gene Aliases: DNMT3A; DNMT3A2; M.HsaIIIA; TBRS
UniProt ID: (Human) Q9Y6K1
Entrez Gene ID: (Human) 1788
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
DNA methylation
methylation-dependent chromatin silencing
regulation of gene expression by genetic imprinting
spermatogenesis
aging
response to toxic substance
response to ionizing radiation
response to lead ion
DNA methylation on cytosine within a CG sequence
positive regulation of cell death
neuron differentiation
response to estradiol
response to vitamin A
response to antidepressant
response to cocaine
DNA methylation involved in embryo development
DNA methylation involved in gamete generation
hypermethylation of CpG island
negative regulation of gene expression, epigenetic
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
cellular response to amino acid stimulus
cellular response to ethanol
cellular response to hypoxia
C-5 methylation of cytosine
hepatocyte apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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