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DOCK11 Antibody (A301-638A) in IP
Detection of human DOCK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK11 antibody A301-638A (lot A301-638A-2) used for IP at 3 µg per reaction. DOCK11 was also immunoprecipitated by rabbit anti-DOCK11 antibody A301-639A. For blotting immunoprecipitated DOCK11, A301-638A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 secon... View More
Product Details
A301-638A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of human dedicator of cytokinesis 11.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Small GTPases of the Rho family, Rho, Rac, and Cdc42, are critical regulators of the actin cytoskeleton and many other cellular processes. Rho GTPases are activated by Dbl-homology (DH)-domain-containing guanine nucleotide exchange factors (GEFs). DOCK 11 (dedicator of cytokinesis 11), also known as ACG or ZIZ2 (zizimin2), is a 2073 amino acid protein belonging to the DOCK family of cytokinesis-regulating proteins that is mainly expressed in peripheral blood leukocytes. DOCK 11 functions as a GEF that binds and activates Cdc42 by exchanging bound GDP for free GTP. Cdc42 mediates cell polarity, gene expression, cell cycle progression and cell-cell contacts. Similar to other DOCK family members, DOCK 11 contains a PH domain and two internal DOCK homology regions designated DHR1 and DHR2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACG; Activated Cdc42-associated guanine nucleotide exchange factor; Activated Cdc42-associated guanine nucleotide exchange factor, ACG; bB128O4.1.1 (novel protein); Dedicator of cytokinesis protein 11; DOCK-11; Zizimin 2; Zizimin-2; zizimin2
Gene Aliases: ACG; bB128O4.1; DOCK11; ZIZ2
UniProt ID: (Human) Q5JSL3
Entrez Gene ID: (Human) 139818
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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