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Invitrogen
DOPA Decarboxylase Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
DOPA Decarboxylase Antibody (PA1-4651) in ICC/IF
Immunofluorescence analysis of DOPA Decarboxylase was performed using 70% confluent log phase SH-SY5Y and SK-O-V3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with DOPA Decarboxylase Polyclonal Antibody (Product # PA1-4651) at 1:100 dilution in 0.1%... View More
Product Details
PA1-4651
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_558672
Product Specific Information
This antibody is specific for the ~55 kDa DDC protein in Western blots of rat adrenal medulla.
Target Information
DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AADC; aromatic L-amino acid decarboxylase; Aromatic-L-amino-acid decarboxylase; DDC; DOPA decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase); HGNC:2719; L-Dopa decarboxylase
Gene Aliases: AADC; DDC
UniProt ID: (Bovine) P27718, (Human) P20711, (Mouse) O88533, (Rat) P14173
Entrez Gene ID: (Bovine) 280762, (Human) 1644, (Mouse) 13195, (Rat) 24311
cellular amino acid metabolic process
dopamine biosynthetic process
circadian rhythm
multicellular organism aging
aminergic neurotransmitter loading into synaptic vesicle
isoquinoline alkaloid metabolic process
cellular response to drug
catecholamine biosynthetic process
serotonin biosynthetic process
indolalkylamine biosynthetic process
response to pyrethroid
phytoalexin metabolic process
cellular response to alkaloid
cellular response to growth factor stimulus
response to toxic substance
carboxylic acid metabolic process
dopamine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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