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Invitrogen
DVL2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
DVL2 Antibody (PA5-17471) in ICC/IF
Immunofluorescence analysis of DVL2 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DVL2 Polyclonal Antibody (Product # PA5-17471) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More
Product Details
PA5-17471
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mink,
Mouse,
Non-human primate,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Ser638 of human Dvl2
Conjugate
Form
Liquid
Concentration
201 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10982147
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with Dvl3.
Target Information
Disheveled DVL2 is a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dishevelled 2; dishevelled 2 (homologous to Drosophila dsh); dishevelled segment polarity protein 2; dishevelled, dsh homolog 2; Dishevelled-2; DSH homolog 2; Segment polarity protein dishevelled homolog DVL-2
Gene Aliases: Dvl-2; DVL2
UniProt ID: (Human) O14641, (Mouse) Q60838
Entrez Gene ID: (Human) 1856, (Chinese hamster) 100762587, (Mouse) 13543, (Rat) 303251
neural tube closure
positive regulation of protein phosphorylation
outflow tract morphogenesis
transcription from RNA polymerase II promoter
segment specification
heart development
Wnt signaling pathway
convergent extension involved in neural plate elongation
cellular protein localization
hippo signaling
non-canonical Wnt signaling pathway
positive regulation of JUN kinase activity
positive regulation of GTPase activity
canonical Wnt signaling pathway involved in regulation of cell proliferation
positive regulation of transcription, DNA-templated
positive regulation of sequence-specific DNA binding transcription factor activity
protein oligomerization
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
positive regulation of protein tyrosine kinase activity
negative regulation of canonical Wnt signaling pathway
cochlea morphogenesis
planar cell polarity pathway involved in neural tube closure
positive regulation of canonical Wnt signaling pathway
beta-catenin destruction complex disassembly
heart morphogenesis
multicellular organism development
segmentation
intracellular signal transduction
convergent extension involved in organogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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