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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin
FIGURE: 1 / 9
DYKDDDDK Tag Antibody (MA1-91878-BTIN) in WB
Western blot analysis was performed on HeLa cells transfected with DYKDDDDK-tagged -BNIP3 plasmid (Lane 1) and untransfected HeLa cells (Lane 2). The blot was probed with Anti-DYKDDDDK Tag Mouse Monoclonal Antibody (FG4R), BIOTIN (Product # MA1-91878-BTIN, 1:500 dilution).A 35 kDa band corresponding to DYKDDDDK tagged BNIP3 was observed in the transfected sample. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) an... View More
Product Details
MA1-91878-BTIN
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
FG4R
Immunogen
Synthetic peptide (DYKDDDDK) coupled to KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537620
Product Specific Information
MA1-91878-BTIN has been successfully used for Western blot, IF and IP applications.
similar epitope as the FLAG tag from Sigma
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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