Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 800 4X PEG
FIGURE: 1 / 8
DYKDDDDK Tag Antibody (MA1-91878-D800) in WB
Western blot analysis of FLAG® Epitope Tag was performed by loading various amounts of E. coli lysate containing a FLAG-epitope tagged protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. F... View More
Product Details
MA1-91878-D800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
FG4R
Immunogen
Synthetic peptide (DYKDDDDK) coupled to KLH.
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
MES with proprietary stabilizer
Contains
no preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537625
Product Specific Information
MA1-91878-D800 detects FLAG tag from various samples. MA1-91878-D800 has been successfully used for fluorescent detection of FLAG tagged proteins in Western Blotting.
Similar epitope as FLAG tag from Sigma.
DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support