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Desmoglein 3 Antibody (PA5-121194) in WB
Western blot analysis of Desmoglein 3 in extracts of various cell lines. Samples were incubated with Desmoglein 3 Polyclonal antibody (Product # PA5-121194) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-121194
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 749-999 of human Desmoglein 3 (NP_001935.2).
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914766
Product Specific Information
Positive test controls include: HeLa, Mouse liver. The target is usually found in the following locations: Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome.
Immunogen sequence: FGAATGVGIC SSGQSGTMRT RHSTGGTNKD YADGAISMNF LDSYFSQKAF ACAEEDDGQE ANDCLLIYDN EGADATGSPV GSVGCCSFIA DDLDDSFLDS LGPKFKKLAE ISLGVDGEGK EVQPPSKDSG YGIESCGHPI EVQQTGFVKC QTLSGSQGAS ALSTSGSVQP AVSIPDPLQH GNYLVTETYS ASGSLVQPST AGFDPLLTQN VIVTERVICP ISSVPGNLAG PTQLRGSHTM LCTEDPCSRL I
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa pemphigus vulgaris antigen; Cadherin family member 6; Desmoglein-3; DKFZp686P23184; PVA
Gene Aliases: CDHF6; DSG3; PVA
UniProt ID: (Human) P32926
Entrez Gene ID: (Human) 1830
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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