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Invitrogen
Desmoglein 3 Recombinant Mouse Monoclonal Antibody (AK23)
FIGURE: 1 / 2
Desmoglein 3 Antibody (MA5-51877) in ICC/IF
Immunocytochemistry analysis of Desmoglein 3 in paraformaldehyde fixed A431 cells using Desmoglein 3 Recombinant Mouse Monoclocal Antibody (Product # MA5-51877) at a 1:100 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: primary antibody, DAPI, merged channels and an isotyp... View More
Product Details
MA5-51877
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
AK23
Immunogen
The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freund's adjuvant subcutaneously.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; Cadherin family member 6; Desmoglein-3; DKFZp686P23184; PVA
Gene Aliases: bal; balding; CDHF6; DSG3; PVA
UniProt ID: (Human) P32926, (Mouse) O35902
Entrez Gene ID: (Human) 1830, (Mouse) 13512
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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