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Dicer Antibody (PA5-115124) in IHC (P)
Immunohistochemistry analysis of Dicer in human normal tissues adjacent to mammary cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115124) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-115124
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DICER1(Accession Q9UPY3), corresponding to amino acid residues K1516-L1566.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899760
Product Specific Information
Antibody detects endogenous levels of total Dicer.
Target Information
Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DCR; DCR-1; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; double-strand-specific ribonuclease mDCR-1; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi
Gene Aliases: 1110006F08Rik; D12Ertd7e; DCR1; DICER; DICER1; Dicer1e; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2
UniProt ID: (Human) Q9UPY3
Entrez Gene ID: (Human) 23405, (Mouse) 192119, (Rat) 299284
double-stranded RNA binding
helicase activity
endoribonuclease activity
ribonuclease III activity
deoxyribonuclease I activity
protein binding
ATP binding
protein domain specific binding
siRNA binding
metal ion binding
pre-miRNA binding
nucleotide binding
nucleic acid binding
RNA binding
nuclease activity
endonuclease activity
hydrolase activity
endoribonuclease activity, producing 5'-phosphomonoesters
miRNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
meiotic spindle organization
angiogenesis
regulation of protein phosphorylation
hair follicle development
apoptotic DNA fragmentation
RNA processing
cell proliferation
regulation of Notch signaling pathway
post-embryonic development
zygote asymmetric cell division
regulation of gene expression
negative regulation of Schwann cell proliferation
positive regulation of gene expression
regulation of muscle cell apoptotic process
positive regulation of Schwann cell differentiation
myoblast differentiation involved in skeletal muscle regeneration
rRNA catabolic process
stem cell population maintenance
spinal cord motor neuron differentiation
nerve development
olfactory bulb interneuron differentiation
cerebral cortex development
lung development
embryonic limb morphogenesis
production of siRNA involved in RNA interference
targeting of mRNA for destruction involved in RNA interference
gene silencing by RNA
pre-miRNA processing
hair follicle morphogenesis
regulation of myelination
positive regulation of myelination
peripheral nervous system myelin formation
conversion of ds siRNA to ss siRNA involved in RNA interference
siRNA loading onto RISC involved in RNA interference
embryonic hindlimb morphogenesis
production of miRNAs involved in gene silencing by miRNA
multicellular organism growth
miRNA loading onto RISC involved in gene silencing by miRNA
conversion of ds siRNA to ss siRNA
regulation of odontogenesis of dentin-containing tooth
regulation of viral genome replication
regulation of cell differentiation
regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
mRNA stabilization
spleen development
digestive tract development
reproductive structure development
regulation of oligodendrocyte differentiation
epidermis morphogenesis
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
anatomical structure development
regulation of neurogenesis
cartilage development
spindle assembly
regulation of RNA metabolic process
defense response to virus
regulation of cell cycle
cardiac muscle cell development
inner ear receptor cell development
negative regulation of glial cell proliferation
intestinal epithelial cell development
cardiac neural crest cell development involved in outflow tract morphogenesis
ganglion development
regulation of enamel mineralization
hair follicle cell proliferation
RNA phosphodiester bond hydrolysis
RNA phosphodiester bond hydrolysis, endonucleolytic
regulation of miRNA metabolic process
positive regulation of miRNA metabolic process
biological_process
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