Invitrogen

Dinitrophenyl-KLH Polyclonal Antibody, Biotin

Dinitrophenyl-KLH Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 1

Dinitrophenyl-KLH Antibody (A-6435) in IHC

Cancers 2021 - Figure 4 HPV viral gene expression levels vary across patients independent of HPV genotype. ( A ) Routine DNA-ISH on papillomas shows HPV+ positive cells predominantly in the more superficial layers of the epithelium. Scale bar, 100 um. HPV6/11 viral E6 and E7 gene expression (white) by RNA-ISH is variable in both ( B ) HPV6+ and ( C ) HPV11+ patient samples; scale bar, 100 um. Two patient samples are shown... View More

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Product Details

A-6435

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Dinitrophenyl-KLH

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536206

Target Information

DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening.

Cancers

Bedard MC,de Alarcon A,Kou YF,Lee D,Sestito A,Duggins AL,Brusadelli M,Lane A,Wikenheiser-Brokamp KA,Wells SI,Smith DF

Published figure using Dinitrophenyl-KLH polyclonal antibody (Product # A-6435) in Immunohistochemistry

Sun May 23 00:00:00 EDT 2021

HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening.

Cancers

Bedard MC,de Alarcon A,Kou YF,Lee D,Sestito A,Duggins AL,Brusadelli M,Lane A,Wikenheiser-Brokamp KA,Wells SI,Smith DF

Published figure using Dinitrophenyl-KLH polyclonal antibody (Product # A-6435) in Immunohistochemistry

Sun May 23 00:00:00 EDT 2021

A universal dual mechanism immunotherapy for the treatment of influenza virus infections.

Nature communications

Liu X,Zhang B,Wang Y,Haymour HS,Zhang F,Xu LC,Srinivasarao M,Low PS

A-6435 was used in Immunocytochemistry-immunoflourescence to design and synthesize a bifunctional small molecule to target the surface of free virus and viral-infected cells in an attempt to treat infections refractory to current therapies.

Thu Nov 05 00:00:00 EST 2020

Oxidative stress, lysosomal damage and dysfunctional autophagy in molluscan hepatopancreas (digestive gland) induced by chemical contaminants.

Marine environmental research

Shaw JP,Moore MN,Readman JW,Mou Z,Langston WJ,Lowe DM,Frickers PE,Al-Moosawi L,Pascoe C,Beesley A

A-6435 was used in Immunocytochemistry-immunofluorescence to test for the induction of dysfunctional autophagy in molluscan hepatopancreatic digestive cells, associated with oxidative stress.

Sun Dec 01 00:00:00 EST 2019

Integration of biochemical, histochemical and toxicogenomic indices for the assessment of health status of mussels from the Tamar Estuary, U.K.

Marine environmental research

Shaw JP,Dondero F,Moore MN,Negri A,Dagnino A,Readman JW,Lowe DR,Frickers PE,Beesley A,Thain JE,Viarengo A

A-6435 was used in western blot to test if a combination of biochemical, histopathological, and toxicogenomic data could be used for the assessment of biological risk associated with pollutants within the Tamar River and Estuary.

Fri Jul 01 00:00:00 EDT 2011

American journal of physiology. Lung cellular and molecular physiology

Umstead TM,Freeman WM,Chinchilli VM,Phelps DS

A-6435 was used in ELISA and western blot to assess expression and oxidation in the bronchoalveolar lavage proteins in the rat due to age-related changes

Thu Jan 01 00:00:00 EST 2009

Impaired cutinase secretion in Saccharomyces cerevisiae induces irregular endoplasmic reticulum (ER) membrane proliferation, oxidative stress, and ER-associated degradation.

Applied and environmental microbiology

Sagt CM,Müller WH,van der Heide L,Boonstra J,Verkleij AJ,Verrips CT

A-6435 was used in western blot to show that impaired secretion of CY028 cutinase in S. cerevisiae cells leads to alterations in the endoplasmic reticulum , unfolded protein response, and endoplasmic reticulum-associated degradation.

Wed May 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: C6H3N2O4; DNP-KLH

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Dinitrophenyl-KLH Polyclonal Antibody, Biotin

Dinitrophenyl-KLH Antibody (A-6435) in IHC

Product Details

A-6435

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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