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Invitrogen
Donkey anti-Mouse IgG (H+L) Secondary Antibody, Texas Red
FIGURE: 1 / 1
Mouse IgG (H+L) Secondary Antibody (PA1-28626) in ICC/IF
Cx32L90H shows defects in gap-junction formation and rapid degradation. a COS-7 cells were transfected with the indicated constructs and immunostained for FLAG-tagged Cx32 (magenta), PDI (yellow) as an ER marker, or GM130 (yellow) as a Golgi marker. Nuclei were stained with DAPI (blue). Anti-FLAG immunofluorescence data are depicted as maximum intensity projections from deconvoluted z-stacks, while PDI, GM1... View More
Product Details
PA1-28626
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
1:1,000-1:5,000
Flow Cytometry (Flow)
1:500-1:2500
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
mouse IgG whole molecule
Conjugate
Excitation/Emission Max
595/613 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Purification
IgG fraction
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10983944
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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