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Invitrogen
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine
FIGURE: 1 / 1
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (31685) in ICC/IF
Immunofluorescence analysis of Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, Rhodamine conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Rabbit ... View More
Product Details
31685
Applications
Tested Dilution
Immunohistochemistry (IHC)
1:50 - 1:200
Immunocytochemistry (ICC/IF)
4 µg/mL
Flow Cytometry (Flow)
1:50 - 1:200
Immunoprecipitation (IP)
1:50 - 1:200
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
573/591 nm
View spectra
Form
Lyophilized
Concentration
1.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient
RRID
AB_429712
Target
IgG
Cross Adsorption
Against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat and sheep serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31685 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31685 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein: 0.56 moles Rhodamine per mole IgG. (lot-dependent).
Reconstitute with 0.4 mL of distilled water (1.5 mg/mL after restoration).
Country of Origin: USA
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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