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Invitrogen
Donkey anti-Rabbit IgG (H+L) ReadyProbes™ Secondary Antibody, Alexa Fluor™ 594
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 21
Rabbit IgG (H+L) Secondary Antibody (R37119) in ICC/IF
Immunofluorescence analysis of Donkey anti-Rabbit IgG (H+L) ReadyProbes™ Secondary Antibody, Alexa Fluor 594 (Product # R37119) was performed using MCF 10A (positive model) and A-431 (negative model) in cells stained with Vimentin Polyclonal antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 2% BSA ... View More
Product Details
R37119
Applications
Tested Dilution
Western Blot (WB)
2 drops per mL
Immunocytochemistry (ICC/IF)
2 drops per mL
Flow Cytometry (Flow)
Assay-Dependent
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin
Conjugate
Excitation/Emission Max
590/618 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS with 0.1% BSA
Contains
<0.1% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2556547
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.
This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Like other ReadyProbes reagents, AlexaFluor 594 Doneky anti-Rabbit IgG Antibody comes in a convenient dropper bottle.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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