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Invitrogen
Donkey anti-Rabbit IgG (H+L) Secondary Antibody, WesternDot™ 625
FIGURE: 1 / 2
Rabbit IgG (H+L) Secondary Antibody (W10806) in WB
Simultaneous detection of three proteins on a single blot using Qdot® secondary antibody conjugates. A western blot containing serial dilutions (20-3 µg protein) of lysates from unstimulated (lanes 2-5) and hEGF-stimulated (lanes 6-9) A431 cells was probed with mouse anti-EGFR, rabbit anti-phospho-EGFR, and chicken anti-GAPDH antibodies, followed by (A) WesternDot® 800 goat anti-mouse (pseudocolored blue), ... View More
Product Details
W10806
Applications
Tested Dilution
Western Blot (WB)
1:500
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Rabbit Immunoglobins
Conjugate
Form
Liquid
Purification
purified
Storage buffer
50mM borate, pH 8.3, with 1M betaine
Contains
0.05% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient
RRID
AB_2556788
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly needed with ECL or colorimetric detection. Differently labeled WesternDot antibodies can be applied to a single blot for simultaneous detection of multiple proteins using standard gel or blot imaging platforms (a list of instruments that are compatible with WesternDot detection can be found in the user manual below). Our WesternDot antibodies utilize Qdot nanocrystals enhanced with VIVID technology, making them brighter than the original Qdot reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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