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Alomone Labs, LTD
Dopamine Transporter (DAT) (extracellular) Polyclonal Antibody, FITC
FIGURE: 1 / 2
Dopamine Transporter (DAT) (extracellular) Antibody (AMT-003-F-50UL) in Flow
Cell surface detection of DAT in mouse TK-1 T-cell lymphoma cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).
Product Details
AMT-003-F-50UL
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT, 2nd extracellular loop
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DA transporter; DAT; dopamine transporter 1; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3; solute carrier family 6, member 3
Gene Aliases: DAT; Dat1; Slc6a3
UniProt ID: (Rat) P23977, (Mouse) Q61327
Entrez Gene ID: (Rat) 24898, (Mouse) 13162
protease binding
receptor binding
dopamine transmembrane transporter activity
dopamine:sodium symporter activity
drug binding
monoamine transmembrane transporter activity
protein complex binding
dopamine binding
protein N-terminus binding
protein phosphatase 2A binding
neurotransmitter:sodium symporter activity
protein binding
symporter activity
primary active transporter
neurotransmitter transport
aging
lactation
sensory perception of smell
locomotory behavior
response to iron ion
response to organic cyclic compound
monoamine transport
dopamine transport
adenohypophysis development
response to nicotine
positive regulation of multicellular organism growth
regulation of dopamine metabolic process
response to cocaine
dopamine biosynthetic process
dopamine catabolic process
response to drug
response to ethanol
response to cAMP
prepulse inhibition
ammonium transmembrane transport
cation transmembrane transport
transport
organic cation transport
sodium ion transmembrane transport
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