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Doublecortin Antibody (PA5-28536) in ICC/IF
Immunofluorescent staining of neurons derived from human ESC with a 1:200 dilution of GeneTex anti-doublecortin antibody (Product # PA5-28536). Anti-doublecortin antibody (red) colocalizes with anti-MAP2 antibody (green). Image courtesy of Jente Lu, UC Irvine.
Product Details
PA5-28536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 154 and 441 of Human Doublecortin
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546012
Product Specific Information
Recommended positive controls: Raji, human DCX-transfected 293T.
Predicted reactivity: Mouse (99%), Rat (97%), Chicken (94%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
Gene Aliases: DBCN; DC; DCX; LISX; SCLH; XLIS
UniProt ID: (Human) O43602
Entrez Gene ID: (Human) 1641
neuron migration
nervous system development
central nervous system development
peptidyl-serine phosphorylation
hippocampus development
layer formation in cerebral cortex
pyramidal neuron development
central nervous system projection neuron axonogenesis
intracellular signal transduction
protein autophosphorylation
axon extension
dendrite morphogenesis
response to electrical stimulus
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