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Invitrogen
Doublecortin Recombinant Rabbit Monoclonal Antibody (ARC0380)
FIGURE: 1 / 1
Doublecortin Antibody (MA5-35223) in WB
Western blot analysis of Doublecortin in extracts of various cell lines. Samples were incubated with Doublecortin Monoclonal antibody (Product # MA5-35223) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35223
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0380
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Doublecortin (O43602)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849127
Product Specific Information
Immunogen sequence: MELDFGHFDE RDKTSRNMRG SRMNGLPSPT HSAHCSFYRT RTLQALSNEK KAKKVRFYRN GDRYFKGIVY AVSSDRFRSF DALLADLTRS LSDNINLPQG
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
Gene Aliases: Dbct; Dcn; Dcx
UniProt ID: (Mouse) O88809
Entrez Gene ID: (Rat) 84394, (Mouse) 13193
neuron migration
brain development
central nervous system projection neuron axonogenesis
intracellular signal transduction
axon extension
dendrite morphogenesis
response to electrical stimulus
multicellular organism development
nervous system development
peptidyl-serine phosphorylation
cell differentiation
protein autophosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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