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E Tag Antibody (PA5-81630) in ICC/IF
Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with E Tag Polyclonal Antibody (Product # PA5-81630) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibo... View More
Product Details
PA5-81630
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788823
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Recognize N-terminal and C-terminal E tag in fusion proteins.
Target Information
E tag is an epitope tag.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E epitope tag; GAPVPYPDPLEPR; GAPVPYPDPLEPR tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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