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Invitrogen
E-cadherin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
E-cadherin Antibody (PA5-81393) in IHC (P)
Immunohistochemical analysis of E-cadherin was performed using formalin-fixed paraffin-embedded mouse skin and mouse brain tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius ... View More
Product Details
PA5-81393
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse E-Cadherin/CDH1 protein (Met1-Val709)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788604
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse E-Cadherin/CDH1.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARC-1; cadherin e; Cadherin-1; cadherin-E; CD324; E-cadherin; Ecadherin; Epithelial cadherin; Uvomorulin
Gene Aliases: AA960649; Cdh1; E-cad; Ecad; L-CAM; Um; UVO
UniProt ID: (Mouse) P09803
Entrez Gene ID: (Mouse) 12550
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
sensory perception of sound
single organismal cell-cell adhesion
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
negative regulation of cell-cell adhesion
regulation of protein localization
regulation of water loss via skin
adherens junction organization
positive regulation of transcription factor import into nucleus
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of transcription, DNA-templated
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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