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Invitrogen
E-cadherin Polyclonal Antibody
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E-cadherin Antibody (PA5-95278) in ICC/IF
Immunocytochemistry analysis of E Cadherin using anti-E Cadherin antibody (Product # PA5-95278) . E Cadherin was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-E Cadherin antibody (Product # PA5-95278) overnight at 4°... View More
Product Details
PA5-95278
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human E Cadherin recombinant protein (Position: A286-A703).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807082
Product Specific Information
Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin
Gene Aliases: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO
UniProt ID: (Human) P12830, (Mouse) P09803, (Rat) Q9R0T4
Entrez Gene ID: (Human) 999, (Mouse) 12550, (Rat) 83502
glycoprotein binding
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
gamma-catenin binding
cell adhesion molecule binding
cadherin binding involved in cell-cell adhesion
protein phosphatase binding
protein domain specific binding
metal ion binding
cadherin
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to toxic substance
single organismal cell-cell adhesion
pituitary gland development
negative regulation of cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
adherens junction organization
response to drug
positive regulation of transcription factor import into nucleus
positive regulation of transcription, DNA-templated
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
sensory perception of sound
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
regulation of protein localization
regulation of water loss via skin
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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