Invitrogen

E-cadherin Recombinant Superclonal Antibody (5HCLC)

1 Published Figure
3 References
View all (150) E-cadherin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

710161

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

5HCLC

Immunogen

Peptide corresponding to amino acids 668-677 of human E-cadherin (epithelial)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532597

Product Specific Information

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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Gene Aliases: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO

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UniProt ID: (Human) P12830, (Mouse) P09803

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Entrez Gene ID: (Human) 999, (Mouse) 12550

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Function(s)
glycoprotein binding calcium ion binding protein binding beta-catenin binding ankyrin binding GTPase activating protein binding gamma-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding metal ion binding cadherin
Process(es)
homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development negative regulation of cell-cell adhesion extracellular matrix disassembly extracellular matrix organization neuron projection development adherens junction organization response to drug positive regulation of transcription factor import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion sensory perception of sound calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration
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