Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
E-cadherin Monoclonal Antibody (ECCD-1)
FIGURE: 1 / 1
E-cadherin Antibody (13-1800) in WB
Frontiers in molecular neuroscience 2022 -
No change was found in the protein expression level of G-actin. Protein expression level of Cx26, beta-actin (A) , and gamma-actin (E) in P0 control and P0 KD groups. beta-actin and gamma-actin protein expression level in cochlear of Cx26 KD mouse models. The ratio of G-actin/F-actin in P0 control and P0 KD group (B) respectively. Western blotting F-actin and G-actin in Western blot results of protein expre... View More
Product Details
13-1800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Fish,
Human,
Mouse,
Rat,
Tag
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
ECCD-1
Immunogen
Mouse teratocarcinoma cell line F9.
Conjugate
Form
Lyophilized
Concentration
2.0 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533004
Product Specific Information
Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Wistar rat after immunization with mouse teratocarcinoma cell line F9.
Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.
Cross reactivity: This antibody does not react with human, bovine, chicken or dog E-cadherin.
Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARC-1; cadherin e; Cadherin-1; cadherin-E; CD324; E-cadherin; Ecadherin; Epithelial cadherin; Uvomorulin
Gene Aliases: AA960649; Cdh1; E-cad; Ecad; L-CAM; Um; UVO
UniProt ID: (Mouse) P09803
Entrez Gene ID: (Mouse) 12550
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
sensory perception of sound
single organismal cell-cell adhesion
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
negative regulation of cell-cell adhesion
regulation of protein localization
regulation of water loss via skin
adherens junction organization
positive regulation of transcription factor import into nucleus
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of transcription, DNA-templated
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support