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Invitrogen
E-selectin Monoclonal Antibody (1.2B6)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
E-selectin Antibody (MA1-22165) in ICC/IF
Immunofluorescence analysis of E-selectin was performed using 70% confluent log phase HUVEC cells treated with 100 U/mL TNF-alpha for 3 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with E-selectin Monoclonal Antibody (1.2B6) (Product # MA1-22165) at 1... View More
Product Details
MA1-22165
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1.2B6
Immunogen
Human E-Selectin (ELAM-1)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_558251
Product Specific Information
Not suitable for immunohistochemistry (paraffin).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; selectin E (endothelial adhesion molecule 1)
Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE
UniProt ID: (Human) P16581, (Pig) P98110
Entrez Gene ID: (Human) 6401, (Pig) 397508
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
inflammatory response
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
activation of phospholipase C activity
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
cell adhesion
positive regulation of leukocyte tethering or rolling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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