Proteintech

E-selectin /CD62E Polyclonal Antibody

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Antibody Testing Data (8)

E-selectin /CD62E Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

E-selectin /CD62E Antibody (20894-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using (Product # 20894-1-AP) E-selectin/CD62E antibody at dilution of 1:4,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

E-selectin /CD62E Antibody in Western Blot (WB)

E-selectin /CD62E Antibody in Flow Cytometry (Flow)

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Product Details

20894-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E-selectin /CD62E Fusion Protein Ag14944 (22-371 aa encoded by BC142711)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: WSYNTSTEA MTYDEASAYC QQRYTHLVAI QNKEEIEYLN SILSYSPSYY WIGIRKVNNV WVWVGTQKPL TEEAKNWAPG EPNNRQKDED CVEIYIKREK DVGMWNDERC SKKKLALCYT AACTNTSCSG HGECVETINN YTCKCDPGFS GLKCEQIVNC TALESPEHGS LVCSHPLGNF SYNSSCSISC DRGYLPSSME TMQCMSSGEW SAPIPACNVV ECDAVTNPAN GFVECFQNPG SFPWNTTCTF DCEEGFELMG AQSLQCTSSG NWDNEKPTCK AVTCRAVRQP QNGSVRCSHS PAGEFTFKSS CNFTCEEGFM LQGPAQVECT TQGQWTQQIP VCEAFQCTAL S (22-371 aa encoded by BC142711)

Target Information

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inflammation Control and Tumor Growth Inhibition of Ovarian Cancer by Targeting Adhesion Molecules of E-Selectin.

Cancers

Yang B,Yin S,Zhou Z,Huang L,Xi M

Tue Apr 04 00:00:00 EDT 2023

Aconitum carmichaelii Debx. Attenuates Heart Failure through Inhibiting Inflammation and Abnormal Vascular Remodeling.

International journal of molecular sciences

Xing Z,Chen J,Yu T,Li X,Dong W,Peng C,Li D

Sun Mar 19 00:00:00 EDT 2023

Insulin Resistance Triggers Atherosclerosis: Caveolin 1 Cooperates with PKCzeta to Block Insulin Signaling in Vascular Endothelial Cells.

Cardiovascular drugs and therapy

Tan J,Li X,Dou N

Thu Jun 08 00:00:00 EDT 2023

Disturbed Flow-Facilitated Margination and Targeting of Nanodisks Protect against Atherosclerosis.

Small (Weinheim an der Bergstrasse, Germany)

Zhao CR,Li J,Jiang ZT,Zhu JJ,Zhao JN,Yang QR,Yao W,Pang W,Li N,Yu M,Gan Y,Zhou J

Sun Jan 01 00:00:00 EST 2023

Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance.

Research square

James J,Dekan A,Niihori M,McClain N,Varghese M,Bharti D,Lawal OS,Padilla-Rodrigez M,Yi D,Dai Z,Gusev O,Rafikova O,Rafikov R

Wed May 03 00:00:00 EDT 2023

Cardamonin targets KEAP1/NRF2 signaling for protection against atherosclerosis.

Food & function

Fan P,Meng H,Hao W,Zheng Y,Li H,Zhang Z,Du L,Guo X,Wang D,Wang Y,Wu H

Mon May 22 00:00:00 EDT 2023

3-(3-Hydroxyphenyl)propionic acid, a microbial metabolite of quercetin, inhibits monocyte binding to endothelial cells via modulating E-selectin expression.

Fitoterapia

Feng J,Ge C,Li W,Li R

Sat Jan 01 00:00:00 EST 2022

Effects of 2,2',4'-trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells.

Oncology letters

Sun JL,Cao ZQ,Sun SW,Sun ZH,Sun SH,Ye JF,Leng P

Fri Apr 01 00:00:00 EDT 2022

The mechanism underlying ICAM-1 and E-selectin-mediated hypertriglyceridemic pancreatitis-associated lung injury.

Molecular immunology

Yu R,Hou C,Peng Y,Zhu X,Shi C,Huang D,Miao Y,Li Q

Thu Dec 01 00:00:00 EST 2022

The mechanism underlying ICAM-1 and E-selectin-mediated hypertriglyceridemic pancreatitis-associated lung injury.

Molecular immunology

Yu R,Hou C,Peng Y,Zhu X,Shi C,Huang D,Miao Y,Li Q

Thu Dec 01 00:00:00 EST 2022

MAC mediates mammary duct epithelial cell injury in plasma cell mastitis and granulomatous mastitis.

International immunopharmacology

Zhang HJ,Ding PP,Zhang XS,Wang XC,Sun DW,Bu QA,Li XQ

Thu Dec 01 00:00:00 EST 2022

Glycyrrhizic Acid Alleviates Lipopolysaccharide (LPS)-Induced Acute Lung Injury by Regulating Angiotensin-Converting Enzyme-2 (ACE2) and Caveolin-1 Signaling Pathway.

Inflammation

Chen Y,Qu L,Li Y,Chen C,He W,Shen L,Zhang R

Tue Feb 01 00:00:00 EST 2022

KLF2 alleviates endothelial cell injury and inhibits the formation of THP‑1 macrophage‑derived foam cells by activating Nrf2 and enhancing autophagy.

Experimental and therapeutic medicine

Tan Z,Ren H,Liu Y,Yang H,Luo Q,Deng X

Thu Dec 01 00:00:00 EST 2022

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Journal of neuroinflammation

Gao C,Jia W,Xu W,Wu Q,Wu J

Sat May 22 00:00:00 EDT 2021

MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Genomics

Shi Y,He W,Zhong M,Yu M

Thu Jul 01 00:00:00 EDT 2021

Reducing Inflammation and Vascular Invasion in Intervertebral Disc Degeneration via Cystathionine-γ-Lyase Inhibitory Effect on E-Selectin.

Frontiers in cell and developmental biology

Xu H,Wei K,Tu J,Chen Y,He Y,Ding Y,Xu H,Bao X,Xie H,Fang H,Wang H

Tue Dec 07 00:00:00 EST 2021

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

International journal of molecular sciences

Morani F,Bisceglia L,Rosini G,Mutti L,Melaiu O,Landi S,Gemignani F

Mon Mar 08 00:00:00 EST 2021

Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion.

Frontiers in cellular and infection microbiology

Zou SS,Zou QC,Xiong WJ,Cui NY,Wang K,Liu HX,Lou WJ,Higazy D,Zhang YG,Cui M

Tue Oct 19 00:00:00 EDT 2021

Monocyte-derived extracellular vesicles upon treated by palmitate promote endothelial migration and monocytes attachment to endothelial cells.

Biochemical and biophysical research communications

Gao W,Guo X,Wang Y,Jian D,Li M

Thu Mar 12 00:00:00 EDT 2020

DNA damage/cGAS-triggered up-regulation of MALAT1 promotes undesirable inflammatory responses in radiotherapy of cancer.

Biochemical and biophysical research communications

Yuan C,Wang D,Zhang N,Wang Z,Yang F,He J,Sun R,Yang X,Hu J,Wu M

Thu Aug 06 00:00:00 EDT 2020

Ketamine attenuates high-glucose-mediated endothelial inflammation in human umbilical vein endothelial cells.

Canadian journal of physiology and pharmacology

Wang T,Zhu H,Hou Y,Duan W,Meng F,Liu Y

Sun Mar 01 00:00:00 EST 2020

Ketamine ameliorates hypoxia-induced endothelial injury in human umbilical vein endothelial cells.

Clinics (Sao Paulo, Brazil)

Zhou X,Liu J,Yang S,Su Y,Meng Z,Hu Y

Thu Oct 15 00:00:00 EDT 2020

The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.

Science advances

Cui H,Zuo S,Liu Z,Liu H,Wang J,You T,Zheng Z,Zhou Y,Qian X,Yao H,Xie L,Liu T,Sham PC,Yu Y,Li MJ

Thu Oct 01 00:00:00 EDT 2020

VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.

Frontiers in cell and developmental biology

Zhu JJ,Jiang ZT,Liu C,Xi YF,Wang J,Yang FF,Yao WJ,Pang W,Han LL,Zhang YH,Sun AQ,Zhou J

Tue Nov 24 00:00:00 EST 2020

Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway.

Journal of cardiovascular pharmacology and therapeutics

Zhao S,Liang M,Wang Y,Hu J,Zhong Y,Li J,Huang K,Li Y

Wed May 01 00:00:00 EDT 2019

Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway.

Journal of cardiovascular pharmacology and therapeutics

Zhao S,Liang M,Wang Y,Hu J,Zhong Y,Li J,Huang K,Li Y

Wed May 01 00:00:00 EDT 2019

MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis.

Cell death & disease

Su Y,Yuan J,Zhang F,Lei Q,Zhang T,Li K,Guo J,Hong Y,Bu G,Lv X,Liang S,Ou J,Zhou J,Luo B,Shang J

Tue May 07 00:00:00 EDT 2019

Gender Difference in Damage-Mediated Signaling Contributes to Pulmonary Arterial Hypertension.

Antioxidants & redox signaling

Rafikov R,Nair V,Sinari S,Babu H,Sullivan JC,Yuan JX,Desai AA,Rafikova O

Fri Nov 01 00:00:00 EDT 2019

Ticagrelor and clopidogrel suppress NF-κB signaling pathway to alleviate LPS-induced dysfunction in vein endothelial cells.

BMC cardiovascular disorders

Jia Z,Huang Y,Ji X,Sun J,Fu G

Mon Dec 30 00:00:00 EST 2019

Propofol attenuates the adhesion of tumor and endothelial cells through inhibiting glycolysis in human umbilical vein endothelial cells.

Acta biochimica et biophysica Sinica

Qi J,Wu Q,Zhu X,Zhang S,Chen X,Chen W,Sun Z,Zhu M,Miao C

Mon Nov 18 00:00:00 EST 2019

SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells.

Acta biochimica et biophysica Sinica

Chen X,Wu Q,Jiang H,Wang J,Zhao Y,Xu Y,Zhu M

Sun Jul 01 00:00:00 EDT 2018

SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells.

Acta biochimica et biophysica Sinica

Chen X,Wu Q,Jiang H,Wang J,Zhao Y,Xu Y,Zhu M

Sun Jul 01 00:00:00 EDT 2018

An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury.

Biomaterials science

Hu JB,Liu D,Qi J,Lu KJ,Jin FY,Ying XY,You J,Du YZ

Tue Nov 20 00:00:00 EST 2018

Toll-Like Receptor 3 Signaling Contributes to Regional Neutrophil Recruitment in Cultured Human Glomerular Endothelial Cells.

Nephron

Liu Q,Imaizumi T,Kawaguchi S,Aizawa T,Matsumiya T,Watanabe S,Tsugawa K,Yoshida H,Tsuruga K,Joh K,Kijima H,Tanaka H

Fri Sep 27 00:00:00 EDT 2019

The BCR/ABL tyrosine kinase inhibitor, nilotinib, stimulates expression of IL-1β in vascular endothelium in association with downregulation of miR-3p.

Leukemia research

Sukegawa M,Wang X,Nishioka C,Pan B,Xu K,Ohkawara H,Hamasaki Y,Mita M,Nakamura K,Okamoto M,Shimura H,Ohta M,Ikezoe T

Sat Jul 01 00:00:00 EDT 2017

The BCR/ABL tyrosine kinase inhibitor, nilotinib, stimulates expression of IL-1β in vascular endothelium in association with downregulation of miR-3p.

Leukemia research

Sukegawa M,Wang X,Nishioka C,Pan B,Xu K,Ohkawara H,Hamasaki Y,Mita M,Nakamura K,Okamoto M,Shimura H,Ohta M,Ikezoe T

Sat Jul 01 00:00:00 EDT 2017

CD40 ligand induces expression of vascular cell adhesion molecule 1 and E-selectin in orbital fibroblasts from patients with Graves' orbitopathy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Wang H,Zhu LS,Cheng JW,Cai JP,Li Y,Ma XY,Wei RL

Wed Apr 01 00:00:00 EDT 2015

CD40 ligand induces expression of vascular cell adhesion molecule 1 and E-selectin in orbital fibroblasts from patients with Graves' orbitopathy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Wang H,Zhu LS,Cheng JW,Cai JP,Li Y,Ma XY,Wei RL

Wed Apr 01 00:00:00 EDT 2015

A hot water extract of Curcuma longa inhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells.

Bioscience, biotechnology, and biochemistry

Kawasaki K,Muroyama K,Yamamoto N,Murosaki S

Thu May 19 00:00:00 EDT 2016

Dynamic shear stress regulation of inflammatory and thrombotic pathways in baboon endothelial outgrowth cells.

Tissue engineering. Part A

Ankeny RF,Hinds MT,Nerem RM

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2

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Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE

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UniProt ID: (Human) P16581

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Entrez Gene ID: (Human) 6401

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Function(s)

transmembrane signaling receptor activity protein binding sialic acid binding phospholipase binding oligosaccharide binding

Process(es)

positive regulation of receptor internalization leukocyte migration involved in inflammatory response inflammatory response heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion activation of phospholipase C activity calcium-mediated signaling actin filament-based process response to lipopolysaccharide response to tumor necrosis factor regulation of inflammatory response leukocyte migration leukocyte tethering or rolling response to interleukin-1

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E-selectin /CD62E Polyclonal Antibody

E-selectin /CD62E Antibody (20894-1-AP) in IHC (P)

Product Details

20894-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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