Proteintech

E2F1 Monoclonal Antibody (5D7G8)

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E2F1 Antibody (66515-1-IG) in WB

WB result of E2F1 antibody (Product # 66515-1-Ig) 1:1,000, incubated at room temperature for 1.5 hours with sh-Control and sh-E2F1 transfected HepG2 cells.

Product Image Gallery

Product Details

66515-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3,000

View 24 publications 24 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 3 publications 3 publications

ChIP assay (ChIP)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5D7G8

Immunogen

E2F1 Fusion Protein Ag17363

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N-Acetyl-L-Cysteine Reduces Cervical Carcinogenesis by Promoting Apoptosis.

Drugs in R&D

Guo W,Jing W

Thu Jun 01 00:00:00 EDT 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer.

The Journal of biological chemistry

Su Y,Zeng K,Liu S,Wu Y,Wang C,Wang S,Lin L,Zou R,Sun G,Luan R,Zhou B,Bai Y,Niu J,Zhang Y,Zhao Y

Sun Jan 01 00:00:00 EST 2023

TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression.

BMC cancer

Wang H,Cui H,Yang X,Peng L

Mon Mar 20 00:00:00 EDT 2023

FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression.

Cell reports

Zhang Y,He B,Zhang D,Zhang Y,Chen C,Zhang W,Yang S,Yao M,Cui G,Gu J,Wang T,Lin Z,Fan Y,Xiong Z,Hao Y

Tue Mar 28 00:00:00 EDT 2023

NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1.

Cancer science

He J,Gao R,Yang J,Li F,Fu Y,Cui J,Liu X,Huang K,Guo Q,Zhou Z,Wei W

Wed Mar 01 00:00:00 EST 2023

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Kim H,Villareal LB,Liu Z,Haneef M,Falcon DM,Martin DR,Lee HJ,Dame MK,Attili D,Chen Y,Varani J,Spence JR,Kovbasnjuk O,Colacino JA,Lyssiotis CA,Lin HC,Shah YM,Xue X

Sat Apr 01 00:00:00 EDT 2023

E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis.

Clinical epigenetics

Li J,Wang J,Wang Y,Zhao X,Su T

Tue Jan 31 00:00:00 EST 2023

E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis.

Clinical epigenetics

Li J,Wang J,Wang Y,Zhao X,Su T

Tue Jan 31 00:00:00 EST 2023

Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation.

Acta pharmacologica Sinica

Xu YJ,Zeng K,Ren Y,Mao CY,Ye YH,Zhu XT,Sun ZY,Cao BY,Zhang ZB,Xu GQ,Huang ZQ,Mao XL

Fri Sep 01 00:00:00 EDT 2023

Inhibition of Angiogenesis by MiR-524-5p through Suppression of AKT and ERK Activation by Targeting CXCR7 in Colon Cancer Cells.

Journal of oncology

Li X,Li Z,Li C,Fang Y,Zhang G,Yan Y,Zhao X,Song X,Zhang Z

Tue Nov 22 00:00:00 EST 2022

Analysis of regulating activities of 5'-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo.

Frontiers in pharmacology

Wang X,Luo X,Gan X,Chen C,Yang Z,Wen J,Fang W,Huang H,Gao C,Zhou X,Feng X,Liu Y

Tue Aug 30 00:00:00 EDT 2022

Icaritin inhibits CDK2 expression and activity to interfere with tumor progression.

iScience

Zhang C,Wang X,Zhang C

Fri Sep 16 00:00:00 EDT 2022

Icaritin inhibits CDK2 expression and activity to interfere with tumor progression.

iScience

Zhang C,Wang X,Zhang C

Fri Sep 16 00:00:00 EDT 2022

POLD2 is activated by E2F1 to promote triple-negative breast cancer proliferation.

Frontiers in oncology

Zhang Z

Tue Sep 20 00:00:00 EDT 2022

POLD2 is activated by E2F1 to promote triple-negative breast cancer proliferation.

Frontiers in oncology

Zhang Z

Tue Sep 20 00:00:00 EDT 2022

Long non-coding RNA SNHG1 activates glycolysis to promote hepatocellular cancer progression through the miR-326/PKM2 axis.

The journal of gene medicine

Wang Y,Yang F,Peng Q,Mei K,He H,Yang Q

Mon Aug 01 00:00:00 EDT 2022

U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells.

Bioengineered

Li Y,Chen S,Zhang X,Zhuo N

Fri Apr 01 00:00:00 EDT 2022

Thymidine Kinase 1 Drives Skin Cutaneous Melanoma Malignant Progression and Metabolic Reprogramming.

Frontiers in oncology

Zuo S,Wang H,Li L,Pan H,Lu L

Tue Mar 22 00:00:00 EDT 2022

Integrating network pharmacology deciphers the action mechanism of Zuojin capsule in suppressing colorectal cancer.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fan JH,Xu MM,Zhou LM,Gui ZW,Huang L,Li XG,Ye XL

Tue Feb 01 00:00:00 EST 2022

Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling.

Frontiers in physiology

Fan S,Wang C,Huang K,Liang M

Wed Feb 23 00:00:00 EST 2022

High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.

Apoptosis : an international journal on programmed cell death

Chen CH,Chou YT,Yang YW,Lo KY

Wed Dec 01 00:00:00 EST 2021

FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression.

Cell biology international

Tang Y,Jiang L,Zhao X,Hu D,Zhao G,Luo S,Du X,Tang W

Wed Dec 01 00:00:00 EST 2021

FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression.

Cell biology international

Tang Y,Jiang L,Zhao X,Hu D,Zhao G,Luo S,Du X,Tang W

Wed Dec 01 00:00:00 EST 2021

FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression.

Cell biology international

Tang Y,Jiang L,Zhao X,Hu D,Zhao G,Luo S,Du X,Tang W

Wed Dec 01 00:00:00 EST 2021

FGF14-AS2 accelerates tumorigenesis in glioma by forming a feedback loop with miR-320a/E2F1 axis.

Journal of Cancer

Zhang P,Gu X,Zhang N,Liu L,Dong X,Li H,Cheng S,Li S,Yuan J,Li Y,Dong J

Fri Oct 22 00:00:00 EDT 2021

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Ecotoxicology and environmental safety

Li Z,Yin H,Shen Y,Ren M,Xu X

Mon Mar 15 00:00:00 EDT 2021

Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma.

Journal of cellular and molecular medicine

Cui XH,Peng QJ,Li RZ,Lyu XJ,Zhu CF,Qin XH

Wed Dec 01 00:00:00 EST 2021

Deletion of p16 accelerates fracture healing in geriatric mice.

American journal of translational research

Ding Q,Liu H,Liu L,Ma C,Qin H,Wei Y,Ren Y

Thu Nov 18 00:00:00 EST 2021

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5.

Frontiers in cell and developmental biology

Xu F,Xiao Z,Fan L,Ruan G,Cheng Y,Tian Y,Chen M,Chen D,Wei Y

Sat Oct 30 00:00:00 EDT 2021

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5.

Frontiers in cell and developmental biology

Xu F,Xiao Z,Fan L,Ruan G,Cheng Y,Tian Y,Chen M,Chen D,Wei Y

Sat Oct 30 00:00:00 EDT 2021

Down-regulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.

The journal of gene medicine

Wu J,Li Y,Liu J,Xu Y

Mon Mar 01 00:00:00 EST 2021

MicroRNA miR-188-5p as a mediator of long non-coding RNA MALAT1 regulates cell proliferation and apoptosis in multiple myeloma.

Bioengineered

Liu H,Chi Z,Jin H,Yang W

Wed Dec 01 00:00:00 EST 2021

Gypenoside LI arrests the cell cycle of breast cancer in G0/G1 phase by down-regulating E2F1.

Journal of ethnopharmacology

Zu ML,Duan Y,Xie JB,Qi YS,Xie P,Borjigidai A,Piao XL

Sat Jun 12 00:00:00 EDT 2021

E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.

Cell death & disease

Yu Y,Zhao D,Li K,Cai Y,Xu P,Li R,Li J,Chen X,Chen P,Cui G

Fri Apr 24 00:00:00 EDT 2020

Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.

FEBS open bio

Oku Y,Nishiya N,Tazawa T,Kobayashi T,Umezawa N,Sugawara Y,Uehara Y

Fri Jun 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1

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Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3

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UniProt ID: (Human) Q01094, (Mouse) Q61501, (Rat) O09139

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Entrez Gene ID: (Human) 1869, (Mouse) 13555, (Rat) 399489

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Function(s)

core promoter binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding sequence-specific DNA binding transcription regulatory region DNA binding RNA metabolism protein nucleic acid metabolism protein general transcription factor

Process(es)

DNA damage checkpoint negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest spermatogenesis intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of gene expression forebrain development anoikis negative regulation of DNA binding negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation mRNA stabilization negative regulation of fat cell proliferation cellular response to fatty acid cellular response to hypoxia cellular response to xenobiotic stimulus negative regulation of transcription involved in G1/S transition of mitotic cell cycle intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway lens fiber cell apoptotic process cellular response to nerve growth factor stimulus regulation of G1/S transition of mitotic cell cycle apoptotic process cell cycle regulation of cell cycle G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E2F1 Monoclonal Antibody (5D7G8)

E2F1 Antibody (66515-1-IG) in WB

Product Details

66515-1-IG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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