Invitrogen

E2F1 Monoclonal Antibody (KH95)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

17 Published Figures

16 References

View all (59) E2F1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 22

E2F1 Antibody (32-1400) in WB

Western blot analysis of E2F-1 was performed by loading 30 µg of HeLa (lane1), Hep G2 (lane2), A431 (lane3), U2OS (lane4), HCT 116 (lane5) cell tissue lysate using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4°C overnight. E2F-1 was detected at ~47 and 60 kDa using E2F-1 Mouse Monoclonal A... View More

E2F1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

E2F1 Antibody in Western Blot, ChIP Assay (WB, ChIP)

E2F1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

E2F1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

E2F1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with E2F1 siRNA and reduction of signal was observed in Western Blot using E2F1 monoclonal Antibody (Product # 32-1400). {KD}

Product Details

32-1400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

0.1-1.0 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunoprecipitation (IP)

2-5 µg

View 3 publications 3 publications

ChIP assay (ChIP)

2 µg/10^6 cells

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KH95

Immunogen

Recombinant human E2F-1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533065

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC.

Frontiers in oncology

Dong X,Zhang Q,Hao J,Xie Q,Xu B,Zhang P,Lu H,Huang Q,Yang T,Wei GH,Na R,Gao P

32-1400 was used in Chromatin immunoprecipitation and Western Blotting to investigate the roles of noncoding variations coordinated with androgen signaling and oncogenic transcription factors in mis-regulating TERT expression and driving PCa.

Sat Dec 04 00:00:00 EST 2021

Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC.

Frontiers in oncology

Dong X,Zhang Q,Hao J,Xie Q,Xu B,Zhang P,Lu H,Huang Q,Yang T,Wei GH,Na R,Gao P

Sat Dec 04 00:00:00 EST 2021

Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC.

Frontiers in oncology

Dong X,Zhang Q,Hao J,Xie Q,Xu B,Zhang P,Lu H,Huang Q,Yang T,Wei GH,Na R,Gao P

Sat Dec 04 00:00:00 EST 2021

MDM2's dual mRNA binding domains co-ordinate its oncogenic and tumour suppressor activities.

Nucleic acids research

Gnanasundram SV,Malbert-Colas L,Chen S,Fusée L,Daskalogianni C,Muller P,Salomao N,Fåhraeus R

32-1400 was used in Immunoprecipitation to illustrate how two MDM2 domains selectively bind specific mRNAs in response to cellular conditions to promote, or suppress, cell growth and how p14Arf coordinates MDM2's activity towards p53 and E2F1.

Thu Jul 09 00:00:00 EDT 2020

MDM2's dual mRNA binding domains co-ordinate its oncogenic and tumour suppressor activities.

Nucleic acids research

Gnanasundram SV,Malbert-Colas L,Chen S,Fusée L,Daskalogianni C,Muller P,Salomao N,Fåhraeus R

Thu Jul 09 00:00:00 EDT 2020

MDM2's dual mRNA binding domains co-ordinate its oncogenic and tumour suppressor activities.

Nucleic acids research

Gnanasundram SV,Malbert-Colas L,Chen S,Fusée L,Daskalogianni C,Muller P,Salomao N,Fåhraeus R

Thu Jul 09 00:00:00 EDT 2020

Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins.

Molecular pharmacology

Choiniere J,Wu J,Wang L

321400 was used in western blot to look for potential tumor suppressors and examine their function as cell cycle modulators and investigate their impact on the cyclin family of proteins and cyclin dependent kinases

Wed Mar 01 00:00:00 EST 2017

Triptolide abrogates growth of colon cancer and induces cell cycle arrest by inhibiting transcriptional activation of E2F.

Laboratory investigation; a journal of technical methods and pathology

Oliveira A,Beyer G,Chugh R,Skube SJ,Majumder K,Banerjee S,Sangwan V,Li L,Dawra R,Subramanian S,Saluja A,Dudeja V

32-1400 was used in immunoprecipitation to study the effect of triptolide on colon cancer growth and cell cycle arrest and its mechanism.

Mon Jun 01 00:00:00 EDT 2015

Triptolide abrogates growth of colon cancer and induces cell cycle arrest by inhibiting transcriptional activation of E2F.

Laboratory investigation; a journal of technical methods and pathology

Oliveira A,Beyer G,Chugh R,Skube SJ,Majumder K,Banerjee S,Sangwan V,Li L,Dawra R,Subramanian S,Saluja A,Dudeja V

32-1400 was used in immunoprecipitation to study the effect of triptolide on colon cancer growth and cell cycle arrest and its mechanism.

Mon Jun 01 00:00:00 EDT 2015

Genomic portrait of resectable hepatocellular carcinomas: implications of RB1 and FGF19 aberrations for patient stratification.

Hepatology (Baltimore, Md.)

Ahn SM,Jang SJ,Shim JH,Kim D,Hong SM,Sung CO,Baek D,Haq F,Ansari AA,Lee SY,Chun SM,Choi S,Choi HJ,Kim J,Kim S,Hwang S,Lee YJ,Lee JE,Jung WR,Jang HY,Yang E,Sung WK,Lee NP,Mao M,Lee C,Zucman-Rossi J,Yu E,Lee HC,Kong G

32-1400 was used in immunohistochemistry - paraffin section to identify the prognostic markers of resectable hepatocellular carcinomas

Mon Dec 01 00:00:00 EST 2014

Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.

PloS one

Silva AR,Santos AC,Farfel JM,Grinberg LT,Ferretti RE,Campos AH,Cunha IW,Begnami MD,Rocha RM,Carraro DM,de Bragança Pereira CA,Jacob-Filho W,Brentani H

32-1400 was used in immunohistochemistry - paraffin section to study the clinical manifestation of Alzheimer's disease involving cell-cycle regulation, neuronal death and repair of oxidative DNA damage

Thu Feb 12 00:00:00 EST 2015

Identification of prognostic biomarkers for glioblastomas using protein expression profiling.

International journal of oncology

Jung Y,Joo KM,Seong DH,Choi YL,Kong DS,Kim Y,Kim MH,Jin J,Suh YL,Seol HJ,Shin CS,Lee JI,Kim JH,Song SY,Nam DH

32-1400 was used in immunohistochemistry - paraffin section to identify diagnostic and prognostic markers for glioblastoma

Sun Apr 01 00:00:00 EDT 2012

Histone deacetylase activity in conjunction with E2F-1 and p53 regulates Apaf-1 expression in 661W cells and the retina.

Journal of neuroscience research

Wallace DM,Cotter TG

Published figure using E2F1 monoclonal antibody (Product # 32-1400) in Western Blot

Sun Mar 01 00:00:00 EST 2009

Histone deacetylase activity in conjunction with E2F-1 and p53 regulates Apaf-1 expression in 661W cells and the retina.

Journal of neuroscience research

Wallace DM,Cotter TG

Published figure using E2F1 monoclonal antibody (Product # 32-1400) in Western Blot

Sun Mar 01 00:00:00 EST 2009

Histone deacetylase activity in conjunction with E2F-1 and p53 regulates Apaf-1 expression in 661W cells and the retina.

Journal of neuroscience research

Wallace DM,Cotter TG

Published figure using E2F1 monoclonal antibody (Product # 32-1400) in Western Blot

Sun Mar 01 00:00:00 EST 2009

Impact of E2F-1 expression on clinical outcome of gastric adenocarcinoma patients with adjuvant chemoradiation therapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lee J,Park CK,Park JO,Lim T,Park YS,Lim HY,Lee I,Sohn TS,Noh JH,Heo JS,Kim S,Lim DH,Kim KM,Kang WK

32-1400 was used in immunohistochemistry - paraffin section to assess expression and prognostic significance of E2F-1 and thymidylate synthase in R(0)-resected gastric adenocarcinoma patients

Tue Jan 01 00:00:00 EST 2008

Overexpression of N-Myc rapidly causes acute myeloid leukemia in mice.

Cancer research

Kawagoe H,Kandilci A,Kranenburg TA,Grosveld GC

32-1400 was used in western blot to determine whether N-Myc overexpression is highly oncogenic in mouse myeloid cells

Thu Nov 15 00:00:00 EST 2007

The novel ETS factor TEL2 cooperates with Myc in B lymphomagenesis.

Molecular and cellular biology

Cardone M,Kandilci A,Carella C,Nilsson JA,Brennan JA,Sirma S,Ozbek U,Boyd K,Cleveland JL,Grosveld GC

Tue Mar 01 00:00:00 EST 2005

The novel ETS factor TEL2 cooperates with Myc in B lymphomagenesis.

Molecular and cellular biology

Cardone M,Kandilci A,Carella C,Nilsson JA,Brennan JA,Sirma S,Ozbek U,Boyd K,Cleveland JL,Grosveld GC

Tue Mar 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1

View more View less

Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3

View more View less

UniProt ID: (Human) Q01094, (Mouse) Q61501, (Rat) O09139

View more View less

Entrez Gene ID: (Human) 1869, (Mouse) 13555, (Rat) 399489

View more View less

Function(s)

core promoter binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding sequence-specific DNA binding transcription regulatory region DNA binding RNA metabolism protein nucleic acid metabolism protein general transcription factor

Process(es)

DNA damage checkpoint negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest spermatogenesis intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of gene expression forebrain development anoikis negative regulation of DNA binding negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation mRNA stabilization negative regulation of fat cell proliferation cellular response to fatty acid cellular response to hypoxia cellular response to xenobiotic stimulus negative regulation of transcription involved in G1/S transition of mitotic cell cycle intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway lens fiber cell apoptotic process cellular response to nerve growth factor stimulus regulation of G1/S transition of mitotic cell cycle apoptotic process cell cycle regulation of cell cycle G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

E2F1 Monoclonal Antibody (KH95)

E2F1 Antibody (32-1400) in WB

Product Details

32-1400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!