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EAAT2 Polyclonal Antibody

EAAT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EAAT2 Antibody (22515-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human brain using 22515-1-AP (EAAT2 antibody) at dilution of 1:50 (under 40x lens).

EAAT2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22515-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EAAT2 Fusion Protein Ag18247 (460-574 aa encoded by BC132768)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PTEDISLLV AVDWLLDRMR TSVNVVGDSF GAGIVYHLSK SELDTIDSQH RVHEDIEMTK TQSIYDDMKN HRESNSNQCV YAAHNSVIVD ECKVTLAANG KSADCSVEEE PWKREK (460-574 aa encoded by BC132768)

Target Information

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Repeated sevoflurane exposures inhibit neurogenesis by inducing the upregulation of glutamate transporter 1 in astrocytes.

The European journal of neuroscience

Kong F,Zhang Y,Wang T,Zhong L,Feng C,Wu Y

Sun Jan 01 00:00:00 EST 2023

Repeated sevoflurane exposures inhibit neurogenesis by inducing the upregulation of glutamate transporter 1 in astrocytes.

The European journal of neuroscience

Kong F,Zhang Y,Wang T,Zhong L,Feng C,Wu Y

Sun Jan 01 00:00:00 EST 2023

ONO-2506 Can Delay Levodopa-induced Dyskinesia in the Early Stage.

Neuroscience

Yuan Y,Zhang X,Wu Y,Lian P,Cao X,Xu Y

Sat Jul 15 00:00:00 EDT 2023

ONO-2506 Can Delay Levodopa-induced Dyskinesia in the Early Stage.

Neuroscience

Yuan Y,Zhang X,Wu Y,Lian P,Cao X,Xu Y

Sat Jul 15 00:00:00 EDT 2023

Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity.

Neural regeneration research

Li KX,Lu M,Cui MX,Wang XM,Zheng Y

Sun Oct 01 00:00:00 EDT 2023

Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

JCI insight

Sha L,Li G,Zhang X,Lin Y,Qiu Y,Deng Y,Zhu W,Xu Q

Mon Aug 08 00:00:00 EDT 2022

Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

JCI insight

Sha L,Li G,Zhang X,Lin Y,Qiu Y,Deng Y,Zhu W,Xu Q

Mon Aug 08 00:00:00 EDT 2022

Cyanidin Chloride Improves LPS-Induced Depression-Like Behavior in Mice by Ameliorating Hippocampal Inflammation and Excitotoxicity.

ACS chemical neuroscience

Qu D,Ye Z,Zhang W,Dai B,Chen G,Wang L,Shao X,Xiang A,Lu Z,Shi J

Wed Nov 02 00:00:00 EDT 2022

Cyanidin Chloride Improves LPS-Induced Depression-Like Behavior in Mice by Ameliorating Hippocampal Inflammation and Excitotoxicity.

ACS chemical neuroscience

Qu D,Ye Z,Zhang W,Dai B,Chen G,Wang L,Shao X,Xiang A,Lu Z,Shi J

Wed Nov 02 00:00:00 EDT 2022

Quantitative proteomic analysis of cortex in the depressive-like behavior of rats induced by the simulated complex space environment.

Journal of proteomics

Min R,Chen Z,Wang Y,Deng Z,Zhang Y,Deng Y

Thu Apr 15 00:00:00 EDT 2021

Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson's Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota.

Oxidative medicine and cellular longevity

Zhou X,Lu J,Wei K,Wei J,Tian P,Yue M,Wang Y,Hong D,Li F,Wang B,Chen T,Fang X

Tue Mar 01 00:00:00 EST 2022

Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression.

Oxidative medicine and cellular longevity

Zhang D,Shen Q,Wu X,Xing D

Wed Jun 09 00:00:00 EDT 2021

Functional validation of a human GLUD2 variant in a murine model of Parkinson's disease.

Cell death & disease

Zhang W,Gong J,Ding L,Zhang Z,Pan X,Chen X,Guo W,Zhang X,Yang X,Peng G,Lin Y,Gao F,Li Y,Zhu X,Xuan A,Wang S,Sun X,Zhang Y,Xu P

Thu Oct 22 00:00:00 EDT 2020

RAGE Signaling pathway in hippocampus dentate gyrus involved in GLT-1 decrease induced by chronic unpredictable stress in rats.

Brain research bulletin

Yang F,Wang H,Chen H,Ran D,Tang Q,Weng P,Sun Y,Jiang W

Thu Oct 01 00:00:00 EDT 2020

Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity.

Theranostics

Sha L,Chen T,Deng Y,Du T,Ma K,Zhu W,Shen Y,Xu Q

Wed May 05 00:00:00 EDT 2021

Ginsenoside Rb1 prevents MPTP-induced changes in hippocampal memory via regulation of the α-synuclein/PSD-95 pathway.

Aging

Qu S,Meng X,Liu Y,Zhang X,Zhang Y

Thu Apr 04 00:00:00 EDT 2019

Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission.

Neurochemical research

Chen S,Zeng X,Zong W,Wang X,Chen L,Zhou L,Li C,Huang Q,Huang X,Zeng G,Hu K,Ouyang DS

Fri Feb 01 00:00:00 EST 2019

HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity.

Aging cell

Yu C,Narasipura SD,Richards MH,Hu XT,Yamamoto B,Al-Harthi L

Sun Oct 01 00:00:00 EDT 2017

HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity.

Aging cell

Yu C,Narasipura SD,Richards MH,Hu XT,Yamamoto B,Al-Harthi L

Sun Oct 01 00:00:00 EDT 2017

Baicalin protects neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the phosphoinositide 3-kinase/protein kinase B signaling pathway.

Neural regeneration research

Zhou ZQ,Li YL,Ao ZB,Wen ZL,Chen QW,Huang ZG,Xiao B,Yan XH

Sun Oct 01 00:00:00 EDT 2017

Baicalin protects neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the phosphoinositide 3-kinase/protein kinase B signaling pathway.

Neural regeneration research

Zhou ZQ,Li YL,Ao ZB,Wen ZL,Chen QW,Huang ZG,Xiao B,Yan XH

Sun Oct 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2

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Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2

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UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596

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Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482

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Function(s)

L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding amino acid transmembrane transporter activity glutamate:sodium symporter activity symporter activity sodium:dicarboxylate symporter activity primary active transporter

Process(es)

amino acid transmembrane transport ion transport chemical synaptic transmission visual behavior response to wounding multicellular organism aging glutamate secretion telencephalon development adult behavior cellular response to extracellular stimulus multicellular organism growth response to drug response to amino acid positive regulation of glucose import L-glutamate import D-aspartate import L-glutamate transmembrane transport transport dicarboxylic acid transport nervous system development response to light stimulus L-glutamate transport sodium ion transmembrane transport neurotransmitter transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EAAT2 Polyclonal Antibody

EAAT2 Antibody (22515-1-AP) in IHC (P)

Product Details

22515-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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