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EDEM3 Antibody (BS-14502R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with EDEM3 Polyclonal Antibody, Unconjugated (bs-14502R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-14502R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human EDEM3, amino acids 401-500.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1,2-mannosidase EDEM3; ER degradation enhancer, mannosidase alpha-like 3; ER degradation-enhancing -mannosidase-like protein 3; ER degradation-enhancing alpha-mannosidase-like 3; ER degradation-enhancing alpha-mannosidase-like protein 3
Gene Aliases: 2310050N11Rik; C1orf22; EDEM3; RGD1561496
UniProt ID: (Human) Q9BZQ6, (Mouse) Q2HXL6
Entrez Gene ID: (Human) 80267, (Rat) 289085, (Mouse) 66967
alpha-mannosidase activity
glycoprotein endo-alpha-1,2-mannosidase activity
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
calcium ion binding
lytic transglycosylase activity
mannosidase activity
mannosyl-oligosaccharide mannosidase activity
galactosidase activity
glucosidase activity
trehalase activity
fucosidase activity
hexosaminidase activity
amylase activity
hydrolase activity
dextrin alpha-glucosidase activity
starch alpha-glucosidase activity
beta-glucanase activity
beta-6-sulfate-N-acetylglucosaminidase activity
glucan endo-1,4-beta-glucosidase activity
glycoprotein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
N-glycan processing
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
endoplasmic reticulum mannose trimming
mannose trimming involved in glycoprotein ERAD pathway
response to unfolded protein
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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