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EDF1 Antibody (CF810789) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EDF1 (RC201996, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-EDF1. (1. Positive lysates LY418427 (100 µg) and LC418427 (20 µg) can be purchased separately from OriGene.
Product Details
CF810789
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7D9
Immunogen
Full length human recombinant protein of human EDF1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein shares significant similarity with Arabidopsis FUS6, which is a regulator of light-mediated signal transduction in plant cells. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calmodulin-associated peptide 19; calmodulin-associated peptide-19; CAP-19; EDF-1; Endothelial differentiation-related factor 1; endothelial differentiation-related factor 1 homolog; hypothetical protein 1-9; MBF1; MGC9058; multiprotein bridging factor 1; Multiprotein-bridging factor 1
Gene Aliases: 0610008L11Rik; AA409425; CAP-19; CFAP280; EDF-1; EDF1; MBF1
UniProt ID: (Human) O60869, (Rat) P69736, (Mouse) Q9JMG1
Entrez Gene ID: (Human) 8721, (Rat) 296570, (Mouse) 59022
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
positive regulation of DNA binding
regulation of nucleic acid-templated transcription
regulation of lipid metabolic process
endothelial cell differentiation
positive regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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