Invitrogen

EEA1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (32) EEA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

EEA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

EEA1 Antibody (PA5-17228) in ICC/IF

Immunofluorescence analysis of EEA1 was performed using 70% confluent log phase BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with EEA1 Polyclonal Antibody (Product # PA5-17228) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790),... View More

EEA1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

EEA1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A-431 cells were transfected with EEA1 siRNA and a decrease in the signal intensity was observed in Western Blot application using Anti-EEA1 Polyclonal Antibody (Product # PA5-17228). {KD}

Product Details

PA5-17228

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

1:25

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding Gly14 of human EEA1 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

8 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11004515

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lipid nanoparticle delivery limits antisense oligonucleotide activity and cellular distribution in the brain after intracerebroventricular injection.

Molecular therapy. Nucleic acids

Byrnes AE,Dominguez SL,Yen CW,Laufer BI,Foreman O,Reichelt M,Lin H,Sagolla M,Hötzel K,Ngu H,Soendergaard C,Estevez A,Lin HC,Goyon A,Bian J,Lin J,Hinz FI,Friedman BA,Easton A,Hoogenraad CC

PA5-17228 was used in Immunocytochemistry-immunofluorescence to provide insights into how LNP encapsulation affects ASO distribution as well as activity in the brain, and a foundation that enables future optimization of brain-targeting ASO-LNPs.

Tue Jun 13 00:00:00 EDT 2023

Disruption of Endosomal Sorting in Schwann Cells Leads to Defective Myelination and Endosomal Abnormalities Observed in Charcot-Marie-Tooth Disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

McLean JW,Wilson JA,Tian T,Watson JA,VanHart M,Bean AJ,Scherer SS,Crossman DK,Ubogu E,Wilson SM

PA5-17228 was used in Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to suggest that defective endosomal trafficking of internalized cell surface receptors may be a common mechanism contributing to demyelinating CMT.

Wed Jun 22 00:00:00 EDT 2022

Membrane Dynamics and Organization of the Phagocyte NADPH Oxidase in PLB-985 Cells.

Frontiers in cell and developmental biology

Joly J,Hudik E,Lecart S,Roos D,Verkuijlen P,Wrona D,Siler U,Reichenbach J,Nüsse O,Dupré-Crochet S

PA5-17228 was used in Immunocytochemistry-immunoflourescence to suggest a role for p47phox and possibly ROS production in NOX2 recruitment at the phagosome.

Thu Dec 31 00:00:00 EST 2020

Membrane Dynamics and Organization of the Phagocyte NADPH Oxidase in PLB-985 Cells.

Frontiers in cell and developmental biology

Joly J,Hudik E,Lecart S,Roos D,Verkuijlen P,Wrona D,Siler U,Reichenbach J,Nüsse O,Dupré-Crochet S

PA5-17228 was used in Immunocytochemistry-immunoflourescence to suggest a role for p47phox and possibly ROS production in NOX2 recruitment at the phagosome.

Thu Dec 31 00:00:00 EST 2020

The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.

Molecular and cellular neurosciences

Gireud-Goss M,Reyes S,Tewari R,Patrizz A,Howe MD,Kofler J,Waxham MN,McCullough LD,Bean AJ

PA5-17228 was used in Western Blot, Immunocytochemistry to suggest that ESCRT components critical for membrane protein sorting in the endocytic pathway are altered in AD.

Thu Oct 01 00:00:00 EDT 2020

The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.

Molecular and cellular neurosciences

Gireud-Goss M,Reyes S,Tewari R,Patrizz A,Howe MD,Kofler J,Waxham MN,McCullough LD,Bean AJ

PA5-17228 was used in Western Blot, Immunocytochemistry to suggest that ESCRT components critical for membrane protein sorting in the endocytic pathway are altered in AD.

Thu Oct 01 00:00:00 EDT 2020

Sensory Axon Growth Requires Spatiotemporal Integration of CaSR and TrkB Signaling.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Markworth R,Adolfs Y,Dambeck V,Steinbeck LM,Lizé M,Pasterkamp RJ,Bähr M,Dean C,Burk K

PA5-17228 was used in Immunocytochemistry-immunoflourescence to investigate receptor signaling in sensory axon growth.

Wed Jul 24 00:00:00 EDT 2019

Distinct mechanisms enable inward or outward budding from late endosomes/multivesicular bodies.

Experimental cell research

Gireud-Goss M,Reyes S,Wilson M,Farley M,Memarzadeh K,Srinivasan S,Sirisaengtaksin N,Yamashita S,Tsunoda S,Lang FF,Waxham MN,Bean AJ

PA5-17228 was used in Western Blotting to observe that inward and outward budding from the MVB membrane are mechanistically distinct but may be linked, such that inhibition of inward budding triggers a re-routing of cargo from inward to outward budding vesicles, without affecting the number of vesicles that bud outwardly from MVBs.

Thu Nov 01 00:00:00 EDT 2018

EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons.

Scientific reports

Burk K,Murdoch JD,Freytag S,Koenig M,Bharat V,Markworth R,Burkhardt S,Fischer A,Dean C

Published figure using EEA1 polyclonal antibody (Product # PA5-17228) in Immunofluorescence

Fri May 19 00:00:00 EDT 2017

EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons.

Scientific reports

Burk K,Murdoch JD,Freytag S,Koenig M,Bharat V,Markworth R,Burkhardt S,Fischer A,Dean C

Published figure using EEA1 polyclonal antibody (Product # PA5-17228) in Immunofluorescence

Fri May 19 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2

View more View less

Gene Aliases: A430109M19Rik; B230358H09Rik; EEA1; MST105; MSTP105; ZFYVE2

View more View less

UniProt ID: (Human) Q15075, (Mouse) Q8BL66

View more View less

Entrez Gene ID: (Human) 8411, (Rat) 314764, (Mouse) 216238

View more View less

Function(s)

protein binding calmodulin binding 1-phosphatidylinositol binding zinc ion binding GTP-dependent protein binding protein homodimerization activity metal ion binding membrane trafficking regulatory protein

Process(es)

endocytosis vesicle fusion synaptic vesicle to endosome fusion early endosome to late endosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

EEA1 Polyclonal Antibody

EEA1 Antibody (PA5-17228) in ICC/IF

Product Details

PA5-17228

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!