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EEFSEC Antibody (PA5-109676) in WB
Western blot analysis of EEFSEC in extracts of various cell lines. Samples were incubated with EEFSEC Polyclonal antibody (Product # PA5-109676) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-109676
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 347-596 of human EEFSEC (NP_068756.2).
Conjugate
Form
Liquid
Concentration
4.64 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855087
Product Specific Information
Immunogen sequence: RLMFFSPAPD NFDQEPILDS FNFSQEYLFQ EQYLSKDLTP AVTDNDEADK KAGQATEGHC PRQQWALVEF EKPVTCPRLC LVIGSRLDAD IHTNTCRLAF HGILLHGLED RNYADSFLPR LKVYKLKHKH GLVERAMDDY SVIGRSLFKK ETNIQLFVGL KVHLSTGELG IIDSAFGQSG KFKIHIPGGL SPESKKILTP ALKKRARAGR GEATRQEESA ERSEPSQHVV LSLTFKRYVF DTHKRMVQSP
Target Information
SELB is a translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elongation factor for selenoprotein translation; Elongation factor sec; Eukaryotic elongation factor, selenocysteine-tRNA-specific; selenocysteine (Sec)-specific eukaryotic elongation factor; Selenocysteine-specific elongation factor
Gene Aliases: EEFSEC; EFSEC; SELB
UniProt ID: (Human) P57772
Entrez Gene ID: (Human) 60678
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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