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EGF Antibody (500-P277BT-25UG) in ELISA
Sandwich ELISA: To detect Rat EGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of EGF Polyclonal Antibody, Biotin (Product # 500-P277BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech EGF Polyclonal Antibody (Product # 500-P277-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat EGF.
Product Details
500-P277BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat EGF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930210
Product Specific Information
AA Sequence of recombinant protein: MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat EGF. Anti-Rat EGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat EGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat EGF (500-P277) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat EGF.
Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P277BT-1MG will be provided as 2 x 500 µg
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EGF; H-EGF; Pro-epidermal growth factor
Gene Aliases: Egf
UniProt ID: (Rat) P07522
Entrez Gene ID: (Rat) 25313
activation of MAPKK activity
angiogenesis
epidermal growth factor receptor signaling pathway
STAT protein import into nucleus
positive regulation of cell proliferation
positive regulation of peptidyl-threonine phosphorylation
peptidyl-tyrosine phosphorylation
positive regulation of cerebellar granule cell precursor proliferation
positive regulation of catenin import into nucleus
intracellular signal transduction
positive regulation of phosphorylation
positive regulation of DNA binding
positive regulation of MAP kinase activity
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
branching morphogenesis of an epithelial tube
regulation of protein secretion
regulation of peptidyl-tyrosine phosphorylation
negative regulation of secretion
regulation of protein transport
mammary gland alveolus development
ERK1 and ERK2 cascade
regulation of calcium ion import
negative regulation of cholesterol efflux
positive regulation of hyaluronan biosynthetic process
regulation of protein localization to cell surface
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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