Invitrogen

EGFR Monoclonal Antibody (225)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

29 References

View all (279) EGFR antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

EGFR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

EGFR Antibody (MA5-12880) in ICC/IF

Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Mouse monoclonal antibody (Product # MA5-12880) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a... View More

EGFR Antibody in Immunoprecipitation (IP)

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EGFR Antibody — Altered expression of target protein upon Knockout demonstrates antibody specificity. Immunofluorescence analysis of EGFR using Anti-EGFR Mouse monoclonal Antibody (Product # MA5-12875) shows no expression in A-431 EGFR knockout cells. {KO}

Product Details

MA5-12880

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 10 publications 10 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 9 publications 9 publications

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

225

Immunogen

Purified EGFR from A431 cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982150

Product Specific Information

MA5-12880 targets Epidermal Growth Factor Receptor in IP and IF applications and shows reactivity with Human and Non-human primate samples.

The MA5-12880 immunogen is purified EGFR from A431 cells.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activating FcγR function depends on endosomal-signaling platforms.

iScience

Benadda S,Nugue M,Koumantou D,Bens M,De Luca M,Pellé O,Monteiro RC,Evnouchidou I,Saveanu L

MA5-12880 was used in Flow Cytometry to investigate here whether endosomal signaling is involved in the function of the human receptors for Fc immunoglobulin fragments (FcRs): FcαRI, FcγRIIA, and FcγRI.

Fri Jul 21 00:00:00 EDT 2023

Litmus-Body: A Molecularly Targeted Sensor for Cell-Surface pH Measurements.

ACS sensors

Kuo JC,Goudge MC,Metzloff AE,Huang LT,Colville MJ,Park S,Zipfel WR,Paszek MJ

MA5-12880 was used in Immunocytochemistry-immunoflourescence to show that the Litmus-body can be successfully targeted to IgG antibodies and provide localized pH measurements in the vicinity of specific cell surface components, as well as following their transit through the endocytic pathway.

Fri Jun 26 00:00:00 EDT 2020

Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.

Molecular cancer therapeutics

Raimbourg J,Joalland MP,Cabart M,de Plater L,Bouquet F,Savina A,Decaudin D,Bennouna J,Vallette FM,Lalier L

MA5-12880 was used in Flow cytometry/Cell sorting to study the effects of cisplatin treatment on the EGFR pathway in non-small cell lung cancer and the functional consequences in animal models of patient-derived xenografts.

Tue Aug 01 00:00:00 EDT 2017

IKKα regulates human keratinocyte migration through surveillance of the redox environment.

Journal of cell science

Lisse TS,Rieger S

MA512880 was used in immunocytochemistry to study H2O2-dependent wound repair mechanisms in human keratinocytes

Wed Mar 01 00:00:00 EST 2017

Interaction of EGFR to δ-catenin leads to δ-catenin phosphorylation and enhances EGFR signaling.

Scientific reports

He Y,Ryu T,Shrestha N,Yuan T,Kim H,Shrestha H,Cho YC,Seo YW,Song WK,Kim K

MA5-12880 was used in immunocytochemistry to study the interaction between EGFR and delta catenin, which leads to delta catenin phosphorylation and EGFR signaling enhancement

Wed Feb 17 00:00:00 EST 2016

Interaction of EGFR to δ-catenin leads to δ-catenin phosphorylation and enhances EGFR signaling.

Scientific reports

He Y,Ryu T,Shrestha N,Yuan T,Kim H,Shrestha H,Cho YC,Seo YW,Song WK,Kim K

MA5-12880 was used in immunocytochemistry to study the interaction between EGFR and delta catenin, which leads to delta catenin phosphorylation and EGFR signaling enhancement

Wed Feb 17 00:00:00 EST 2016

PHD4 stimulates tumor angiogenesis in osteosarcoma cells via TGF-α.

Molecular cancer research : MCR

Klotzsche-von Ameln A,Prade I,Grosser M,Kettelhake A,Rezaei M,Chavakis T,Flamme I,Wielockx B,Breier G

MA5-12880 was used in blocking or activating experiment to study the role of TGF-alpha in the mechanism by which prolyl hydroxylase-4 promotes osteosarcoma cell tumor angiogenesis

Fri Nov 01 00:00:00 EDT 2013

In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse.

International journal of nanomedicine

Terakawa M,Tsunoi Y,Mitsuhashi T

MA5-12880 was used in immunocytochemistry to study the ability of antibody-conjugated biodegradable microspheres excited by a single 80 femtosecond near-IR laser pulse to perforate the cell membrane of epithelial carcinoma cells

Mon Dec 10 00:00:00 EST 2012

Epidermal growth factor receptor as a biomarker for cervical cancer.

Annals of oncology : official journal of the European Society for Medical Oncology

Soonthornthum T,Arias-Pulido H,Joste N,Lomo L,Muller C,Rutledge T,Verschraegen C

MA5-12880 was used in immunohistochemistry to investigate the expression of epidermal growth factor receptor in cervical cancer

Sat Oct 01 00:00:00 EDT 2011

The β-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells.

The international journal of biochemistry & cell biology

Lu R,Bian F,Zhang X,Qi H,Chuang EY,Pflugfelder SC,Li DQ

MA5-12880 was used in immunocytochemistry to study the role of the beta-catenin-Tcf4-survivin signaling pathway in human corneal epithelial progenitor cells

Sun May 01 00:00:00 EDT 2011

An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kojic N,Chung E,Kho AT,Park JA,Huang A,So PT,Tschumperlin DJ

MA5-12880 was used in blocking or activating experiment to investigate lateral intercellular space deformation between bronchial epithelial cells in response to compressive stress

Sat May 01 00:00:00 EDT 2010

The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics.

Journal of endodontics

Kajiya M,Shiba H,Komatsuzawa H,Ouhara K,Fujita T,Takeda K,Uchida Y,Mizuno N,Kawaguchi H,Kurihara H

MA5-12880 was used in blocking or activating experiment to investigate the influence of antimicrobial peptide LL37 on human pulp cell migration

Tue Jun 01 00:00:00 EDT 2010

Nano-bio-chip sensor platform for examination of oral exfoliative cytology.

Cancer prevention research (Philadelphia, Pa.)

Weigum SE,Floriano PN,Redding SW,Yeh CK,Westbrook SD,McGuff HS,Lin A,Miller FR,Villarreal F,Rowan SD,Vigneswaran N,Williams MD,McDevitt JT

MA5-12880 was used in immunocytochemistry to develop a nano-bio-chip sensor to detect biomarkers of early oral tumorigenesis

Thu Apr 01 00:00:00 EDT 2010

Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.

American journal of obstetrics and gynecology

Leach RE,Kilburn BA,Petkova A,Romero R,Armant DR

MA5-12880 was used in blocking or activating experiment to study the role of HB-EGF as a survival factor in human cytotrophoblast cells exposed to hypoxia/reoxygenation injury

Tue Apr 01 00:00:00 EDT 2008

Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.

Biology of reproduction

Wolff GS,Chiang PJ,Smith SM,Romero R,Armant DR

MA5-12880 was used in blocking or activating experiment to examine the anti-apoptotic effect of epidermal growth factor-like growth factors in human placental cytotrophoblast cells

Sun Jul 01 00:00:00 EDT 2007

Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.

Biochemical and biophysical research communications

Yasuda H,Hirata S,Inoue K,Mashima H,Ohnishi H,Yoshiba M

MA5-12880 was used in western blot to investigate the role of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of EGFR and MAPK in gastric carcinoma cells

Fri Mar 02 00:00:00 EST 2007

Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.

Development (Cambridge, England)

Armant DR,Kilburn BA,Petkova A,Edwin SS,Duniec-Dmuchowski ZM,Edwards HJ,Romero R,Leach RE

MA5-12880 was used in blocking or activating experiment to study the role of metalloproteinase-mediated shedding of HB-EGF-like growth factor in human trophoblast survival at low oxygen concentrations

Wed Feb 01 00:00:00 EST 2006

Receptor-selective retinoids inhibit the growth of normal and malignant breast cells by inducing G1 cell cycle blockade.

Breast cancer research and treatment

Wu K,DuPré E,Kim H,Tin-U CK,Bissonnette RP,Lamph WW,Brown PH

MA5-12880 was used in blocking or activating experiment to study the role of G1 cell cycle blockade in the inhibition of normal and cancerous breast cell gowth by synthetic, receptor selctive retionoids

Wed Mar 01 00:00:00 EST 2006

Inhibition of xenograft tumor growth and down-regulation of ErbB receptors by an antibody directed against Lewis Y antigen.

The Journal of pharmacology and experimental therapeutics

Farhan H,Schuster C,Klinger M,Weisz E,Waxenecker G,Schuster M,Sexl V,Mudde GC,Freissmuth M,Kircheis R

MA5-12880 was used in blocking or activating experiment to study the inhibition of xenograft tumor growth and down-regulation of ErbB receptors by an antibody directed against Lewis Y antigen

Fri Dec 01 00:00:00 EST 2006

Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.

Proteins

Kim YS,Bhandari R,Cochran JR,Kuriyan J,Wittrup KD

MA5-12880 was used in flow cytometry to express functional and properly folded EGFR extracellular domain in yeast

Wed Mar 01 00:00:00 EST 2006

The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.

The Journal of biological chemistry

Ward DM,Vaughn MB,Shiflett SL,White PL,Pollock AL,Hill J,Schnegelberger R,Sundquist WI,Kaplan J

MA5-12880 was used in immunocytochemistry to study the role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells

Fri Mar 18 00:00:00 EST 2005

Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display.

Journal of molecular biology

Chao G,Cochran JR,Wittrup KD

MA5-12880 was used in immunoprecipitation to map the epitopes of therapeutically important epidermal growth factor receptor antibodies

Fri Sep 10 00:00:00 EDT 2004

Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.

Molecular biology of the cell

Kansra S,Stoll SW,Johnson JL,Elder JT

MA5-12880 was used in western blot to investigate the mechanism of ERK autocrine activation in normal human keratinocytes

Wed Sep 01 00:00:00 EDT 2004

Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.

Journal of immunological methods

Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD

MA5-12880 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes

Thu Apr 01 00:00:00 EST 2004

Autoantibodies to the epidermal growth factor receptor in systemic sclerosis, lupus, and autoimmune mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Planque S,Zhou YX,Nishiyama Y,Sinha M,O'Connor-Mccourt M,Arnett FC,Paul S

MA5-12880 was used in western blot to study the auto-immune response against epidermal growth factor receptor

Sat Feb 01 00:00:00 EST 2003

Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity.

The Journal of biological chemistry

Planque S,Taguchi H,Burr G,Bhatia G,Karle S,Zhou YX,Nishiyama Y,Paul S

MA5-12880 was used in EMSA to investigate the nucleophilic reactivity of natural antibodies

Fri May 30 00:00:00 EDT 2003

Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jin K,Mao XO,Sun Y,Xie L,Jin L,Nishi E,Klagsbrun M,Greenberg DA

MA5-12880 was used in blocking or activating experiment to study the possible role of HB-EGF in hypoxic-ischemic induction of neurogenesis

Mon Jul 01 00:00:00 EDT 2002

Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking.

The Journal of biological chemistry

Yang J,Kim O,Wu J,Qiu Y

MA5-12880 was used in immunocytochemistry to study the interaction between tyrosine kinase Etk and RUFY1 and the potential role in vesicle trafficking

Fri Aug 16 00:00:00 EDT 2002

Differential utilization and localization of ErbB receptor tyrosine kinases in skin compared to normal and malignant keratinocytes.

Neoplasia (New York, N.Y.)

Stoll SW,Kansra S,Peshick S,Fry DW,Leopold WR,Wiesen JF,Sibilia M,Zhang T,Werb Z,Derynck R,Wagner EF,Elder JT

MA5-12880 was used in immunocytochemistry and immunoprecipitation to study the differential utilization and localization of ErbB receptor tyrosine kinases in skin compared to normal and malignant keratinocytes

Tue Dec 04 00:00:00 EST 2001

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

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Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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Function(s)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EGFR Monoclonal Antibody (225)

EGFR Antibody (MA5-12880) in ICC/IF

Product Details

MA5-12880

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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