Invitrogen

EGFR Monoclonal Antibody (528), Biotin

EGFR Antibody in Immunoprecipitation (IP)

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EGFR Antibody (MA5-12872) in IP

Immunoprecipitation of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-12872) on Native Human A431 Cells.

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Product Details

MA5-12872

Applications

Tested Dilution

Publications

Western Blot (WB)

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 7 publications 7 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 17 publications 17 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

528

Immunogen

Purified EGFR from A431 cells

View immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980304

Product Specific Information

MA5-12872 targets Epidermal Growth Factor Receptor in FACS and IP applications and shows reactivity with Canine and Human samples.

The MA5-12872 immunogen is purified EGFR from A431 cells.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Direct visualization of single-molecule membrane protein interactions in living cells.

PLoS biology

Kim DH,Park S,Kim DK,Jeong MG,Noh J,Kwon Y,Zhou K,Lee NK,Ryu SH

MA5-12872 was used in Flow cytometry/Cell sorting to develop a co-immunoimmobilisation assay enabling the direct observation of membrane protein interactions in single living cells that overcomes the limitations of currently prevalent proximity-based indirect methods.

Sat Dec 01 00:00:00 EST 2018

Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling.

Oncogene

Hasegawa Y,Takahashi M,Ariki S,Asakawa D,Tajiri M,Wada Y,Yamaguchi Y,Nishitani C,Takamiya R,Saito A,Uehara Y,Hashimoto J,Kurimura Y,Takahashi H,Kuroki Y

MA5-12872 was used in immunoprecipitation and western blot to study the role of impaired EGF binding to EGFR in the mechanism by which surfactant protein D inhibits the progression of lung cancer

Thu Feb 12 00:00:00 EST 2015

mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes.

Science signaling

Chou CK,Lee HH,Tsou PH,Chen CT,Hsu JM,Yamaguchi H,Wang YN,Lee HJ,Hsu JL,Lee JF,Kameoka J,Hung MC

MA5-12872 was used in ChIP assay to develop a novel flow-proteomic methodology for the detection of individual signaling complex protein-protein interactions in crude cell/tissue lysates

Tue Mar 04 00:00:00 EST 2014

Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization.

PloS one

Otero C,Linke M,Sanchez P,González A,Schaap IA

MA5-12872 was used in blocking or activating experiment to study the ability of propanolol to restrict cell surface EGFR mobility prior to receptor internalization

Mon Mar 30 00:00:00 EDT 2015

Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands.

PloS one

Henriksen L,Grandal MV,Knudsen SL,van Deurs B,Grøvdal LM

MA5-12872 was used in immunocytochemistry to study internalization of the EGFR following activation by various ligands and the different mechanisms involved

Tue Dec 03 00:00:00 EST 2013

Intracytoplasmic epidermal growth factor receptor shows poor response to the cetuximab antitumor effect in irradiated non-small cell lung cancer cell lines.

Lung cancer (Amsterdam, Netherlands)

Ryu SH,Lee SW,Yang YJ,Song SY,Kim JH,Choi EK,Ahn SD

MA5-12872 was used in flow cytometry to study the anti-tumor activity of a blocking antibody against cytoplasmic EGFR in a non-small cell lung cancer cell line

Sat Sep 01 00:00:00 EDT 2012

The oncoprotein ErbB3 is endocytosed in the absence of added ligand in a clathrin-dependent manner.

Carcinogenesis

Sak MM,Breen K,Rønning SB,Pedersen NM,Bertelsen V,Stang E,Madshus IH

MA5-12872 was used in flow cytometry to study the clathrin-dependent endocytosis of the ErbB3 oncoprotein

Tue May 01 00:00:00 EDT 2012

Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.

Methods in molecular biology (Clifton, N.J.)

Pötz O,Schneiderhan-Marra N,Henzler T,Herget T,Joos TO

MA5-12872 was used in blocking or activating experiment to develop a bead-based sandwich immunoassay for the profiling of receptor tyrosine kinase inhibitors

Thu Jan 26 00:00:00 EST 2012

Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity.

Cell death & disease

Siegemund M,Pollak N,Seifert O,Wahl K,Hanak K,Vogel A,Nussler AK,Göttsch D,Münkel S,Bantel H,Kontermann RE,Pfizenmaier K

MA5-12872 was used in immunoprecipitation to develop targeted dimerized single-chain TRAIL fusion proteins displaying markedly increased anti-tumor activity

Thu Apr 12 00:00:00 EDT 2012

A Miniaturized Ligand Binding Assay for EGFR.

International journal of proteomics

Schwenk JM,Poetz O,Zeillinger R,Joos TO

MA5-12872 was used in ELISA to develop a miniaturized ligand binding assay for EGFR

Thu Aug 23 00:00:00 EDT 2012

EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity.

Cancer letters

Zhu H,Cao X,Ali-Osman F,Keir S,Lo HW

MA5-12872 was used in immunoprecipitation to study the kinase activity-independent binding of EGRF and EGFRvIII to PUMA in glioblastoma cells and the significance for resistance to anti-tumor drugs

Sun Aug 01 00:00:00 EDT 2010

High speed digital protein interaction analysis using microfluidic single molecule detection system.

Lab on a chip

Chou CK,Jing N,Yamaguchi H,Tsou PH,Lee HH,Chen CT,Wang YN,Hong S,Su C,Kameoka J,Hung MC

MA5-12872 was used in western blot to study the use of a microfluidic single molecule detection system for the rapid analysis of protein interactions

Wed Jul 21 00:00:00 EDT 2010

Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.

Journal of cellular physiology

Qi H,Zheng X,Yuan X,Pflugfelder SC,Li DQ

MA5-12872 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium

Fri Oct 01 00:00:00 EDT 2010

IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes.

Experimental dermatology

Poindexter NJ,Williams RR,Powis G,Jen E,Caudle AS,Chada S,Grimm EA

MA5-12872 was used in immunoprecipitation to study the role of IL-24 in wound repair and its inhibitory effect on TGFalpha-induced migration and proliferation of keratinocytes

Sun Aug 01 00:00:00 EDT 2010

Intracellular MUC1 peptides inhibit cancer progression.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bitler BG,Menzl I,Huerta CL,Sands B,Knowlton W,Chang A,Schroeder JA

MA5-12872 was used in immunoprecipitation to study the anti-tumor activity of cell-permeant peptides designed to block MUC1 interactions with beta-catenin and EGFR

Thu Jan 01 00:00:00 EST 2009

EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer.

International journal of cancer

Zhang Y,Su H,Rahimi M,Tochihara R,Tang C

MA5-12872 was used in western blot to study the ability of EGFRvIII to promote estrogen-independent growth in breast cancer cell lines

Sun Nov 01 00:00:00 EDT 2009

LRIG1 negatively regulates the oncogenic EGF receptor mutant EGFRvIII.

Oncogene

Stutz MA,Shattuck DL,Laederich MB,Carraway KL,Sweeney C

MA5-12872 was used in western blot to examine the role of LRIG1 in the degradation of oncogenic EGF receptor mutant EGFRvIII

Thu Sep 25 00:00:00 EDT 2008

Freud-1/Aki1, a novel PDK1-interacting protein, functions as a scaffold to activate the PDK1/Akt pathway in epidermal growth factor signaling.

Molecular and cellular biology

Nakamura A,Naito M,Tsuruo T,Fujita N

MA5-12872 was used in immunoprecipitation to investigate the role of Freud-1/Aki1 in the PDK1/Akt pathway

Wed Oct 01 00:00:00 EDT 2008

Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation.

Molecular biology of the cell

Umebayashi K,Stenmark H,Yoshimori T

MA5-12872 was used in immunoprecipitation to study the role of Ubc4/5 and c-Cbl in the polyubiquitination and degradation of internalized EGFR

Fri Aug 01 00:00:00 EDT 2008

Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt.

Cancer research

Yamasaki F,Johansen MJ,Zhang D,Krishnamurthy S,Felix E,Bartholomeusz C,Aguilar RJ,Kurisu K,Mills GB,Hortobagyi GN,Ueno NT

MA5-12872 was used in immunoprecipitation to study the mechanism of acquired resistance to erlotinib in epidermoid cancer cells

Fri Jun 15 00:00:00 EDT 2007

Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition.

British journal of cancer

Xu W,Soga S,Beebe K,Lee MJ,Kim YS,Trepel J,Neckers L

MA5-12872 was used in immunoprecipitation to study the sensitivity of EGFR and ErbB2 exon 20 insertion mutants to Hsp90 inhibition

Mon Sep 17 00:00:00 EDT 2007

Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.

Proceedings of the National Academy of Sciences of the United States of America

Crotty T,Cai J,Sakane F,Taketomi A,Prescott SM,Topham MK

MA5-12872 was used in immunoprecipitation to study the role of PKC in the mechanism by which diacylglycerol kinase-delta regulates EGFR signaling

Tue Oct 17 00:00:00 EDT 2006

Molecular basis for sensitivity and acquired resistance to gefitinib in HER2-overexpressing human gastric cancer cell lines derived from liver metastasis.

British journal of cancer

Yokoyama H,Ikehara Y,Kodera Y,Ikehara S,Yatabe Y,Mochizuki Y,Koike M,Fujiwara M,Nakao A,Tatematsu M,Nakanishi H

MA5-12872 was used in flow cytometry to study the possibility of molecular therapy targeting HER2 overexpression in gastric cancer liver metastasis

Mon Dec 04 00:00:00 EST 2006

Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1.

Journal of cellular biochemistry

Lo HW,Ali-Seyed M,Wu Y,Bartholomeusz G,Hsu SC,Hung MC

MA5-12872 was used in immunoprecipitation to study the mechanism of EGFR nuclear-cytoplasmic transport

Tue Aug 15 00:00:00 EDT 2006

Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.

Proteins

Kim YS,Bhandari R,Cochran JR,Kuriyan J,Wittrup KD

MA5-12872 was used in flow cytometry to express functional and properly folded EGFR extracellular domain in yeast

Wed Mar 01 00:00:00 EST 2006

Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling.

Molecular pharmacology

Martin NP,Mohney RP,Dunn S,Das M,Scappini E,O'Bryan JP

MA5-12872 was used in immunoprecipitation to study the mechanism by which the endocytic scaffold protein intersectin regulates EGFR degradation and signaling

Wed Nov 01 00:00:00 EST 2006

The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3.

The Journal of biological chemistry

Funes M,Miller JK,Lai C,Carraway KL,Sweeney C

MA5-12872 was used in immunocytochemistry to study the role of ErbB2 and ErbB3 in the mechanism by which Muc4 potentiates neuregulin signaling

Fri Jul 14 00:00:00 EDT 2006

Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor.

Biochemistry

Whitson KB,Whitson SR,Red-Brewer ML,McCoy AJ,Vitali AA,Walker F,Johns TG,Beth AH,Staros JV

MA5-12872 was used in flow cytometry to study the functional effects of N-glycosylation of Asn-579 of EGFR

Tue Nov 15 00:00:00 EST 2005

Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.

Journal of immunological methods

Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD

MA5-12872 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes

Thu Apr 01 00:00:00 EST 2004

Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization.

Proceedings of the National Academy of Sciences of the United States of America

Schmidt MHH,Furnari FB,Cavenee WK,Bögler O

MA5-12872 was used in western blot to study the role of EGFR signaling intensity in determining intracellular protein interactions, ubiquitination, and internalization

Tue May 27 00:00:00 EDT 2003

Suppression of EGFRvIII-mediated proliferation and tumorigenesis of breast cancer cells by ribozyme.

International journal of cancer

Luo X,Gong X,Tang CK

MA5-12872 was used in immunocytochemistry to evaluate the anti-tumor effect of tumor specific ribozyme targeted to EGFRvIII

Sat May 10 00:00:00 EDT 2003

Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture.

The Journal of biological chemistry

Stoll SW,Kansra S,Elder JT

MA5-12872 was used in immunoprecipitation to study the role of MMPs in stimulating ErbB-dependent ERK signaling in human skin organ culture

Fri Jul 26 00:00:00 EDT 2002

1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor.

The Journal of biological chemistry

Cordero JB,Cozzolino M,Lu Y,Vidal M,Slatopolsky E,Stahl PD,Barbieri MA,Dusso A

MA5-12872 was used in immunoprecipitation to study the down regulatory effects of 1,25-dihydroxyvitamin D on EGFR membrane trafficing and growth signaling

Fri Oct 11 00:00:00 EDT 2002

Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.

Journal of the National Cancer Institute

Bergmann-Leitner ES,Bennett TA,Hacker NF,Stromberg K,Stetler-Stevenson WG

MA5-12872 was used in immunoprecipitation to study the role of EGFR signaling in the invasiveness of ovarian carcinoma cells

Wed Sep 19 00:00:00 EDT 2001

Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation.

The Journal of biological chemistry

Gaudry CA,Palka HL,Dusek RL,Huen AC,Khandekar MJ,Hudson LG,Green KJ

MA5-12872 was used in immunoprecipitation to study the effects of EGFR activation on the association of tyrosine phosphorylated plakoglobin with desmogleins and desmoplakin

Fri Jul 06 00:00:00 EDT 2001

Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer.

Cancer research

Tang CK,Gong XQ,Moscatello DK,Wong AJ,Lippman ME

MA5-12872 was used in immunoprecipitation to study the role of the constitutively active EGFR vIII variant in the proliferation and tumorigenicity of human breast cancer

Thu Jun 01 00:00:00 EDT 2000

Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo.

Cancer research

Tang CK,Concepcion XZ,Milan M,Gong X,Montgomery E,Lippman ME

MA5-12872 was used in flow cytometry to study the role of ErbB-4 in the proliferation of estrogen receptor-positive breast cancer cells

Fri Oct 15 00:00:00 EDT 1999

Mitosis-specific negative regulation of epidermal growth factor receptor, triggered by a decrease in ligand binding and dimerization, can be overcome by overexpression of receptor.

The Journal of biological chemistry

Kiyokawa N,Lee EK,Karunagaran D,Lin SY,Hung MC

MA5-12872 was used in immunoprecipitation and western blot to study the mechanism of the mitosis-specific negative regulation of EGFR and the effect receptor overexpression

Fri Jul 25 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

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Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Dog) 404306, (Human) 1956

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Function(s)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor

Process(es)

Performance Guarantee

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EGFR Monoclonal Antibody (528), Biotin

EGFR Antibody (MA5-12872) in IP

Product Details

MA5-12872

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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