Invitrogen

EGFR Monoclonal Antibody (H11)

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

46 References

View all (279) EGFR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

EGFR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

EGFR Antibody (MA1-12693) in ICC/IF

Immunofluorescence analysis of EGFR was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were subsequently labeled with EGFR Mouse Monoclonal Antibody (Product # MA1-12693) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

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Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of EGFR using EGFR Antibody (H11), Mouse Monoclonal (Product # MA1-12693) performed along with another EGFR antibody (Product # MA5-13269) shows similar pattern of EGFR expression in A-431 cells. {IAV}

Product Details

MA1-12693

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mg lysate

View 3 publications 3 publications

Neutralization (Neu)

View 2 publications 2 publications

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

H11

Immunogen

HC2 20 d2 cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074165

Product Specific Information

MA1-12693 detects Epidermal Growth Factor Receptor in human samples.

MA1-12693 has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures. Staining of formalin-fixed tissues requires digestion with protease xxv at 1 mg/mL in PBS for 5 minutes at 37ºC. Positive controls include A431 cells or placenta or squamous cell carcinoma. Suitable for Western blotting of extracellular domain of EGFR.

The MA1-12693 immunogen is HC2 20 d2 cells.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity.

Cell

Jacob F,Salinas RD,Zhang DY,Nguyen PTT,Schnoll JG,Wong SZH,Thokala R,Sheikh S,Saxena D,Prokop S,Liu DA,Qian X,Petrov D,Lucas T,Chen HI,Dorsey JF,Christian KM,Binder ZA,Nasrallah M,Brem S,O'Rourke DM,Ming GL,Song H

MA1-12693 was used in Immunohistochemistry to report methods for generating and biobanking patient-derived glioblastoma organoids (GBOs) that recapitulate the histological features, cellular diversity, gene expression, and mutational profiles of their corresponding parental tumors.

Thu Jan 09 00:00:00 EST 2020

Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR T cell testing.

Nature protocols

Jacob F,Ming GL,Song H

MA1-12693 was used in Immunohistochemistry to develop a procedure for the generation of glioblastoma organoids.

Tue Dec 01 00:00:00 EST 2020

MUC1 stimulates EGFR expression and function in endometrial cancer.

Oncotarget

Engel BJ,Bowser JL,Broaddus RR,Carson DD

MA112693 was used in immunohistochemistry and western blot to determine that MUC1 stimulates epidermal growth factor receptor expression and function in endometrial cancer

Tue May 31 00:00:00 EDT 2016

Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures.

Advanced healthcare materials

Engel BJ,Constantinou PE,Sablatura LK,Doty NJ,Carson DD,Farach-Carson MC,Harrington DA,Zarembinski TI

MA1-12693 was used in immunocytochemistry to use a high-throughput 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells to evaluate chemotherapeutics

Wed Aug 05 00:00:00 EDT 2015

Efficacy and mechanism of action of Proellex, an antiprogestin in aromatase overexpressing and Letrozole resistant T47D breast cancer cells.

The Journal of steroid biochemistry and molecular biology

Gupta A,Mehta R,Alimirah F,Peng X,Murillo G,Wiehle R,Mehta RG

MA1-12693 was used in immunocytochemistry to study the efficacy and anti-prolifferative mechanism of Proellex in breast cancer cells overexpressing aromatase and resistant to Letrozole

Tue Jan 01 00:00:00 EST 2013

Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines.

Annals of oncology : official journal of the European Society for Medical Oncology

Collins DM,O'Donovan N,McGowan PM,O'Sullivan F,Duffy MJ,Crown J

MA1-12693 was used in western blot to study the induction of antibody-dependent cell-mediated cytotoxicity in HER-2-non-amplified breast cancer cells by trastuzumab

Sun Jul 01 00:00:00 EDT 2012

Triepitopic antibody fusions inhibit cetuximab-resistant BRAF and KRAS mutant tumors via EGFR signal repression.

Journal of molecular biology

Spangler JB,Manzari MT,Rosalia EK,Chen TF,Wittrup KD

MA1-12693 was used in blocking or activating experiment to study the therapeutic effects against cetuximab-resistant BRAF and KRAS mutant tumors of an antibody fusion protein that recognizes three EGFR epitopes

Fri Sep 28 00:00:00 EDT 2012

Coexpression of SGLT1 and EGFR is associated with tumor differentiation in oral squamous cell carcinoma.

Odontology

Hanabata Y,Nakajima Y,Morita K,Kayamori K,Omura K

MA1-12693 was used in western blot to investigate the relationship between SGLT1 and EGFR gene expression and tumor differetiation in oral squamous cell carcinoma

Sun Jul 01 00:00:00 EDT 2012

Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action.

Breast cancer research : BCR

DeFazio-Eli L,Strommen K,Dao-Pick T,Parry G,Goodman L,Winslow J

MA1-12693 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors

Fri Apr 15 00:00:00 EDT 2011

Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.

Cytometry. Part B, Clinical cytometry

Eifler RL,Lind J,Falkenhagen D,Weber V,Fischer MB,Zeillinger R

MA1-12693 was used in immunocytochemistry to develop a process involving leukapheresis, elutriation and FACS to isolate circulating tumor cells from large volumes

Tue Mar 01 00:00:00 EST 2011

Determination of the minimal melatonin exposure required to induce osteoblast differentiation from human mesenchymal stem cells and these effects on downstream signaling pathways.

Journal of pineal research

Sethi S,Radio NM,Kotlarczyk MP,Chen CT,Wei YH,Jockers R,Witt-Enderby PA

MA1-12693 was used in western blot to determine the minimal period of melatonin treatment required to induce the differentiation of human mesenchymal stem cells into osteoblasts

Fri Oct 01 00:00:00 EDT 2010

Design of surfaces for liquid crystal-based bioanalytical assays.

ACS applied materials & interfaces

Lowe AM,Ozer BH,Bai Y,Bertics PJ,Abbott NL

MA1-12693 was used in blocking or activating experiment to investigate the liquid crystal-based bioanalytical assays

Mon Mar 01 00:00:00 EST 2010

Activity and cellular localization of an oncogenic glioblastoma multiforme-associated EGF receptor mutant possessing a duplicated kinase domain.

Oncogene

Ozer BH,Wiepz GJ,Bertics PJ

MA1-12693 was used in immunohistochemistry to describe an EGF receptor mutant with tandem kinase domain duplication in glioblastoma multiforme biopsies and cell lines

Thu Feb 11 00:00:00 EST 2010

Evaluation of PTEN and Mcl-1 expressions in NSCLC expressing wild-type or mutated EGFR.

Medical oncology (Northwood, London, England)

Cetin Z,Ozbilim G,Erdogan A,Luleci G,Karauzum SB

MA1-12693 was used in western blot to study the expression of PTEN, Mcl-1 and EGFR in non-small cell lung cancer

Wed Sep 01 00:00:00 EDT 2010

Overexpression of cortactin increases invasion potential in oral squamous cell carcinoma.

Pathology oncology research : POR

Yamada S,Yanamoto S,Kawasaki G,Mizuno A,Nemoto TK

MA1-12693 was used in western blot to study the role of cortactin overexpression in increasing invasion potential in oral squamous cell carcinoma

Wed Dec 01 00:00:00 EST 2010

Erlotinib and bevacizumab have synergistic activity against melanoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Schicher N,Paulitschke V,Swoboda A,Kunstfeld R,Loewe R,Pilarski P,Pehamberger H,Hoeller C

MA1-12693 was used in western blot to study the effect of combined treatment with erlotinib and bevacizumab against melanoma

Fri May 15 00:00:00 EDT 2009

miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Adam L,Zhong M,Choi W,Qi W,Nicoloso M,Arora A,Calin G,Wang H,Siefker-Radtke A,McConkey D,Bar-Eli M,Dinney C

MA1-12693 was used in western blot to study the effect of miR-200 expression on the epithelial-to-mesenchymal transition and resistance to EGFR therapy in bladder cancer cells

Sat Aug 15 00:00:00 EDT 2009

Sennoside B inhibits PDGF receptor signaling and cell proliferation induced by PDGF-BB in human osteosarcoma cells.

Life sciences

Chen YC,Chang CN,Hsu HC,Chiou SJ,Lee LT,Hseu TH

MA1-12693 was used in western blot to study the role of PDGF signaling in the inhibition of osteosarcoma cell proliferation by sennoside B

Fri Jun 19 00:00:00 EDT 2009

Proteomic and immunologic analyses of brain tumor exosomes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Graner MW,Alzate O,Dechkovskaia AM,Keene JD,Sampson JH,Mitchell DA,Bigner DD

MA1-12693 was used in western blot to investigate brain tumor exosomes by biochemical, proteomic, and immunological approaches

Fri May 01 00:00:00 EDT 2009

Sensitivity to epidermal growth factor receptor inhibitor requires E-cadherin expression in urothelial carcinoma cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Black PC,Brown GA,Inamoto T,Shrader M,Arora A,Siefker-Radtke AO,Adam L,Theodorescu D,Wu X,Munsell MF,Bar-Eli M,McConkey DJ,Dinney CP

MA1-12693 was used in western blot to study molecular markers of the response to cetuximab therapy in a panel of urothelial carcinoma cell lines

Sat Mar 01 00:00:00 EST 2008

Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system.

Lab on a chip

Lowe AM,Ozer BH,Wiepz GJ,Bertics PJ,Abbott NL

MA1-12693 was used in immunoprecipitation to develop a novel system for capture and isolation of biomedically important proteins

Fri Aug 01 00:00:00 EDT 2008

Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R).

Cell biochemistry and function

Wu YY,Li H,Lv XY,Wei Q,Li X,Liu XY,Zhou Q,Wei YQ

MA1-12693 was used in western blot to investigate the effect of heterogenous nuclear ribonucleoprotein D-like protein on androgen-independent LNCaP cell proliferation

Sun Jun 01 00:00:00 EDT 2008

Structure-guided development of affinity probes for tyrosine kinases using chemical genetics.

Nature chemical biology

Blair JA,Rauh D,Kung C,Yun CH,Fan QW,Rode H,Zhang C,Eck MJ,Weiss WA,Shokat KM

MA1-12693 was used in western blot to develop tyrosine kinase affinity probes using chemical genetics

Sun Apr 01 00:00:00 EDT 2007

EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer.

Oncogene

Choi SH,Mendrola JM,Lemmon MA

MA1-12693 was used in immunocytochemistry to study EGF-independent activation of mutant cell-surface EGF receptors in gefitinib-sensitive lung cancer

Thu Mar 08 00:00:00 EST 2007

Radiotherapy response in oral squamous carcinoma cell lines: evaluation of apoptotic proteins as prognostic factors.

Head & neck

Roberg K,Jonsson AC,Grénman R,Norberg-Spaak L

MA1-12693 was used in western blot to investigate the effect of apoptotic proteins on the radiosensitivity of oral squamous carcinoma cell line

Sun Apr 01 00:00:00 EDT 2007

Type II combi-molecules: design and binary targeting properties of the novel triazolinium-containing molecules JDD36 and JDE05.

Anti-cancer drugs

Qiu Q,Domarkas J,Banerjee R,Katsoulas A,McNamee JP,Jean-Claude BJ

MA1-12693 was used in western blot to study the EGFR-targeting potential of novel triazolinium-containing "combi-molecules

Thu Feb 01 00:00:00 EST 2007

A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma.

Cancer cell

Fan QW,Knight ZA,Goldenberg DD,Yu W,Mostov KE,Stokoe D,Shokat KM,Weiss WA

MA1-12693 was used in western blot to study the efficacy of a dual PI3 kinase/mTOR inhibitor in glioma

Mon May 01 00:00:00 EDT 2006

Novel toll-like receptor 9 agonist induces epidermal growth factor receptor (EGFR) inhibition and synergistic antitumor activity with EGFR inhibitors.

Clinical cancer research : an official journal of the American Association for Cancer Research

Damiano V,Caputo R,Bianco R,D'Armiento FP,Leonardi A,De Placido S,Bianco AR,Agrawal S,Ciardiello F,Tortora G

MA1-12693 was used in western blot to investigate the effect of a novel toll-like receptor 9 agonist on epidermal growth factor receptor inhibition and on tumor growth

Sun Jan 15 00:00:00 EST 2006

Multiple acquired renal carcinoma tumor capabilities abolished upon silencing of ADAM17.

Cancer research

Franovic A,Robert I,Smith K,Kurban G,Pause A,Gunaratnam L,Lee S

MA1-12693 was used in western blot to study the effect of ADAM17 silencing on multiple acquired renal carcinoma tumor capabilities

Tue Aug 15 00:00:00 EDT 2006

Estrogen receptors in membrane lipid rafts and signal transduction in breast cancer.

Molecular and cellular endocrinology

Márquez DC,Chen HW,Curran EM,Welshons WV,Pietras RJ

MA1-12693 was used in western blot to study the role of estrogen receptors in membrane lipid rafts in breast cancer signal transduction

Sun Feb 26 00:00:00 EST 2006

Uncoupling between epidermal growth factor receptor and downstream signals defines resistance to the antiproliferative effect of Gefitinib in bladder cancer cells.

Cancer research

Kassouf W,Dinney CP,Brown G,McConkey DJ,Diehl AJ,Bar-Eli M,Adam L

MA1-12693 was used in western blot to study the mechanism of resistance to the EGFR tyrosine kinase inhibitor Gefitinib in bladder cancer cells

Tue Nov 15 00:00:00 EST 2005

Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo.

Molecular therapy : the journal of the American Society of Gene Therapy

Saydam O,Glauser DL,Heid I,Turkeri G,Hilbe M,Jacobs AH,Ackermann M,Fraefel C

MA1-12693 was used in western blot to study whether gene silencing by vector-mediated RNAi inhibition of EGFR expression can reduce the growth of cultured human glioma cells

Tue Nov 01 00:00:00 EST 2005

In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.

Cancer cell

García-Echeverría C,Pearson MA,Marti A,Meyer T,Mestan J,Zimmermann J,Gao J,Brueggen J,Capraro HG,Cozens R,Evans DB,Fabbro D,Furet P,Porta DG,Liebetanz J,Martiny-Baron G,Ruetz S,Hofmann F

MA1-12693 was used in ELISA to study the in vivo antitumor activity of a potent and selective IGF-1 receptor kinase inhibitor

Mon Mar 01 00:00:00 EST 2004

Inhibition of the type III epidermal growth factor receptor variant mutant receptor by dominant-negative EGFR-CD533 enhances malignant glioma cell radiosensitivity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lammering G,Hewit TH,Holmes M,Valerie K,Hawkins W,Lin PS,Mikkelsen RB,Schmidt-Ullrich RK

MA1-12693 was used in immunoprecipitation to study the role and therapuetic potential of the EGFR type III in modulating cellular responses to ionizing radiation

Fri Oct 01 00:00:00 EDT 2004

AEE788: a dual family epidermal growth factor receptor/ErbB2 and vascular endothelial growth factor receptor tyrosine kinase inhibitor with antitumor and antiangiogenic activity.

Cancer research

Traxler P,Allegrini PR,Brandt R,Brueggen J,Cozens R,Fabbro D,Grosios K,Lane HA,McSheehy P,Mestan J,Meyer T,Tang C,Wartmann M,Wood J,Caravatti G

MA1-12693 was used in ELISA to suggest that AEE788 is an anticancer agent that deregulates tumor cell proliferation and angiogenic parameters

Thu Jul 15 00:00:00 EDT 2004

Expression of a naturally occurring constitutively active variant of the epidermal growth factor receptor in mouse fibroblasts increases motility.

International journal of cancer

Pedersen MW,Tkach V,Pedersen N,Berezin V,Poulsen HS

MA1-12693 was used in western blot to study the role of a constitutively active variant of the EGFR in increasing mouse fibroblast motility

Fri Feb 20 00:00:00 EST 2004

HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization.

Molecular cancer research : MCR

Dragowska WH,Warburton C,Yapp DT,Minchinton AI,Hu Y,Waterhouse DN,Gelmon K,Skov K,Woo J,Masin D,Huxham LA,Kyle AH,Bally MB

MA1-12693 was used in flow cytometry to study the role of HER-2/neu overexpression in increasing the viable hypoxic cell population within solid tumors

Mon Nov 01 00:00:00 EST 2004

The combi-targeting concept: dissection of the binary mechanism of action of the combi-triazene SMA41 in vitro and antitumor activity in vivo.

The Journal of pharmacology and experimental therapeutics

Matheson SL,McNamee JP,Wang T,Alaoui-Jamali MA,Tari AM,Jean-Claude BJ

MA1-12693 was used in western blot to study the anti-tumor mechanism of a unimolecular combination of an EGFR tyrosine kinase inhibitor and a DNA damaging triazine

Wed Dec 01 00:00:00 EST 2004

Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.

Journal of immunological methods

Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD

MA1-12693 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes

Thu Apr 01 00:00:00 EST 2004

Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity.

The Journal of biological chemistry

Planque S,Taguchi H,Burr G,Bhatia G,Karle S,Zhou YX,Nishiyama Y,Paul S

MA1-12693 was used in EMSA to investigate the nucleophilic reactivity of natural antibodies

Fri May 30 00:00:00 EDT 2003

Neuregulin expression, function, and signaling in human ovarian cancer cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP

MA1-12693 was used in western blot to investigate the characteristics of neuregulin in human ovarian cancer cells

Sun Dec 01 00:00:00 EST 2002

Epidermal growth factor receptor mutation type III transfected into a small cell lung cancer cell line is predominantly localized at the cell surface and enhances the malignant phenotype.

International journal of cancer

Damstrup L,Wandahl Pedersen M,Bastholm L,Elling F,Skovgaard Poulsen H

MA1-12693 was used in immunohistochemistry and western blot to study the role of EGFR mutation type III in enhancing the malignant phenotype in a small cell lung cancer cell line

Tue Jan 01 00:00:00 EST 2002

Inhibition of epidermal growth factor receptor-mediated signaling by "Combi-triazene" BJ2000, a new probe for Combi-Targeting postulates.

The Journal of pharmacology and experimental therapeutics

Brahimi F,Matheson SL,Dudouit F,McNamee JP,Tari AM,Jean-Claude BJ

MA1-12693 was used in western blot to study the inhibition of EGFR-mediated signaling by a "Combi-triazene" molecule with binary EGFR targeting/DNA-damaging properties

Tue Oct 01 00:00:00 EDT 2002

Adenovirus-mediated overexpression of dominant negative epidermal growth factor receptor-CD533 as a gene therapeutic approach radiosensitizes human carcinoma and malignant glioma cells.

International journal of radiation oncology, biology, physics

Lammering G,Lin PS,Contessa JN,Hampton JL,Valerie K,Schmidt-Ullrich RK

MA1-12693 was used in western blot to study the use of adenovirus-mediated overexpression of dominant negative EGFR to radiosensitize human carcinoma and malignant glioma cells

Thu Nov 01 00:00:00 EST 2001

Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCaT-ras transfected cells.

Experimental dermatology

Charvat S,Chignol MC,Souchier C,Le Griel C,Schmitt D,Serres M

MA1-12693 was used in flow cytometry to study the effect of epidermal growth factor and HaCaT-ras on MMP-9 expression and cell migration

Sat Aug 01 00:00:00 EDT 1998

Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells.

The Journal of biological chemistry

Bandyopadhyay D,Mandal M,Adam L,Mendelsohn J,Kumar R

MA1-12693 was used in western blot to study the biological functions of the epidermal growth factor receptor and DNA-dependent protein kinase interaction

Fri Jan 16 00:00:00 EST 1998

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone; waved 2

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Gene Aliases: 9030024J15Rik; AI552599; EGFR; ERBB; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5

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UniProt ID: (Human) P00533, (Mouse) Q01279

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Entrez Gene ID: (Human) 1956, (Mouse) 13649

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Function(s)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding glycoprotein binding protein kinase activity signal transducer activity receptor binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding epidermal growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EGFR Monoclonal Antibody (H11)

EGFR Antibody (MA1-12693) in ICC/IF

Product Details

MA1-12693

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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