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Invitrogen
EGR1 Monoclonal Antibody (T.126.1)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
EGR1 Antibody (MA5-15009) in ICC/IF
Immunofluorescence analysis of EGR1 was performed using PC-12 cells treated with NGF (100 ng/mL for 2 hr) post 12 hr serum starvation. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGR1 Monoclonal Antibody (T.160.5) (Product # MA5-15009) at 1:100 dilution in 0.1% BSA, incubated at 4 °C overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary An... View More
Product Details
MA5-15009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
T.126.1
Immunogen
Synthetic peptide corresponding to the amino terminus of the sequence of human EGR1
Conjugate
Form
Liquid
Concentration
117 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10982091
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AT225; Early growth response protein 1; EGR-1; Krox-24 nuclear protein; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24
Gene Aliases: A530045N19Rik; AT225; egr; Egr-1; EGR1; ETR103; G0S30; Krox-1; KROX-24; KROX24; Ngf1; NGF1-A; Ngfi; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; ZIF-268; Zif268; ZNF225
UniProt ID: (Human) P18146, (Rat) P08154, (Mouse) P08046
Entrez Gene ID: (Human) 1958, (Rat) 24330, (Mouse) 13653
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone acetyltransferase binding
sequence-specific DNA binding
metal ion binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
double-stranded DNA binding
transcription factor binding
transcription regulatory region DNA binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to glucose
T cell differentiation
BMP signaling pathway
response to insulin
regulation of protein sumoylation
skeletal muscle cell differentiation
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
type I interferon signaling pathway
regulation of transcription from RNA polymerase II promoter in response to hypoxia
interleukin-1-mediated signaling pathway
cellular response to gamma radiation
cellular response to heparin
cellular response to mycophenolic acid
glomerular mesangial cell proliferation
positive regulation of glomerular metanephric mesangial cell proliferation
negative regulation of canonical Wnt signaling pathway
response to amphetamine
regulation of transcription, DNA-templated
learning or memory
circadian rhythm
positive regulation of gene expression
positive regulation of cell death
response to nutrient levels
response to follicle-stimulating hormone
cellular response to insulin stimulus
response to carbon monoxide
response to gonadotropin
cellular response to drug
response to cocaine
response to drug
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
response to ethanol
regulation of long-term neuronal synaptic plasticity
oligodendrocyte differentiation
response to electrical stimulus
long-term synaptic potentiation
cellular response to antibiotic
cellular response to mechanical stimulus
cellular response to organic substance
cellular response to isoquinoline alkaloid
cellular response to cAMP
cellular response to growth factor stimulus
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to steroid hormone stimulus
cellular response to hyperoxia
cellular response to hypoxia
response to norepinephrine
transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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