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EGR2 Antibody (PA5-90195) in WB
Western blot analysis of EGR2 in mouse kidney. Samples were incubated with EGR2 Polyclonal antibody (Product # PA5-90195) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-90195
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human EGR2 (NP_0003902)
Conjugate
Form
Liquid
Concentration
1.92 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805956
Product Specific Information
Immunogen sequence: LAYPPPPSYP SPKPATDPGL FPMIPDYPGF FPSQCQRDLH GTAGPDRKPF PCPLDTLRVP PPLTPLSTIR NFTLGGPSAG VTGPGASGGS EGPRLPGSSS A; Positive Samples: mouse kidney; Cellular Location: Nucleus
Target Information
The EGR2 gene provides instructions for making a protein called early growth response 2, which is part of the early growth response family of proteins. These proteins bind to specific areas of DNA and help control the activity of particular genes. On the basis of this action, the proteins are referred to as transcription factors. The early growth response 2 protein activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. Myelin promotes the efficient transmission of nerve impulses. If myelin is lost (demyelination) or its structure is disrupted, the transmission of nerve impulses is impaired. Mutations in the EGR2 gene can cause two forms of Charcot-Marie-Tooth disease, type 1D or type 4E (sometimes called congenital hypomyelinating neuropathy) or a severe form of type 1D (sometimes called Dejerine-Sottas syndrome) that begins during infancy or early childhood.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686J1957; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; Early growth response protein 2; EGR-2; FLJ1454; FLJ14547; Krox-20 homolog; OTTHUMP00000062133; Zinc finger protein Krox-20
Gene Aliases: Egr-2; Egr2; Krox-20; Krox20; NGF1-B; Zfp-25; Zfp-6
UniProt ID: (Mouse) P08152
Entrez Gene ID: (Mouse) 13654
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
nucleic acid binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
ubiquitin protein ligase binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
transcription regulatory region DNA binding
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
HMG box domain binding
C2H2 zinc finger transcription factor
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein export from nucleus
rhythmic behavior
motor neuron axon guidance
Schwann cell differentiation
rhombomere 3 development
facial nerve structural organization
rhombomere 3 formation
rhombomere 5 formation
regulation of ossification
response to insulin
brain segmentation
skeletal muscle cell differentiation
myelination
negative regulation of apoptotic process
fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of neuronal synaptic plasticity
cellular response to organic substance
cellular response to cAMP
cellular response to gonadotropin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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