Invitrogen

EIF2S1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (53) EIF2S1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

EIF2S1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

EIF2S1 Antibody (PA5-27366) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of eIF2a was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2a Polyclonal Antibody (Product # PA5-27366) diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

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Product Details

PA5-27366

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 315 of Human EIF2 alpha

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.63 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544842

Product Specific Information

Recommended positive controls: A549, H1299, HCT-116, rat neuron, HeLa, N2a.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Chicken (97%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA5-27366 was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA5-27366 was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Fisetin Protects HaCaT Human Keratinocytes from Fine Particulate Matter (PM_2.5)-Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response.

Antioxidants (Basel, Switzerland)

Molagoda IMN,Kavinda MHD,Choi YH,Lee H,Kang CH,Lee MH,Lee CM,Kim GY

PA5-27366 was used in Western Blotting to suggest that fisetin inhibits PM2.5-induced apoptosis by inhibiting the ER stress response and production of ROS.

Sat Sep 18 00:00:00 EDT 2021

Fisetin Protects HaCaT Human Keratinocytes from Fine Particulate Matter (PM_2.5)-Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response.

Antioxidants (Basel, Switzerland)

Molagoda IMN,Kavinda MHD,Choi YH,Lee H,Kang CH,Lee MH,Lee CM,Kim GY

PA5-27366 was used in Western Blotting to suggest that fisetin inhibits PM2.5-induced apoptosis by inhibiting the ER stress response and production of ROS.

Sat Sep 18 00:00:00 EDT 2021

ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.

Journal of biomedical science

Cheung CHY,Hsu CL,Lin TY,Chen WT,Wang YC,Huang HC,Juan HF

Published figure using EIF2S1 polyclonal antibody (Product # PA5-27366) in Western Blot

Tue Jun 23 00:00:00 EDT 2020

ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.

Journal of biomedical science

Cheung CHY,Hsu CL,Lin TY,Chen WT,Wang YC,Huang HC,Juan HF

Published figure using EIF2S1 polyclonal antibody (Product # PA5-27366) in Western Blot

Tue Jun 23 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A

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Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1

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UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6

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Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665

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Function(s)

translation initiation factor activity protein binding ribosome binding poly(A) RNA binding nucleic acid binding RNA binding translation initiation factor

Process(es)

positive regulation of defense response to virus by host translational initiation aging negative regulation of translational initiation in response to stress cellular response to amino acid starvation cellular response to heat cellular response to UV response to endoplasmic reticulum stress PERK-mediated unfolded protein response protein autophosphorylation transmembrane transport mitophagy in response to mitochondrial depolarization xenophagy positive regulation of neuron death response to manganese-induced endoplasmic reticulum stress positive regulation of type B pancreatic cell apoptotic process translation regulation of translational initiation in response to stress regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2S1 Polyclonal Antibody

EIF2S1 Antibody (PA5-27366) in ICC/IF

Product Details

PA5-27366

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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