Invitrogen

EIF2S1 Monoclonal Antibody (EIF2-alpha)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

22 Published Figures

20 References

View all (53) EIF2S1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

EIF2S1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

EIF2S1 Antibody (AHO0802) in ICC/IF

Immunofluorescence analysis of eIF2a was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with eIF2a Mouse Monoclonal Antibody at 1:250 dilution in1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for... View More

EIF2S1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EIF2S1 Antibody in Immunoprecipitation (IP)

EIF2S1 Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

Product Details

AHO0802

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

EIF2-alpha

Immunogen

Recombinant human eIF-2-alpha.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536316

Product Specific Information

This antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (EIF2-alpha). Recommended positive control are human Jurkat, CEM, and HeLa cells; mouse 3T3L1 cells; and rat PC-12 cells.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer.

Cancer science

Wang SF,Chang YL,Fang WL,Li AF,Chen CF,Yeh TS,Hung GY,Huang KH,Lee HC

Published figure using EIF2S1 monoclonal antibody (Product # AHO0802) in Western Blot

Tue Aug 01 00:00:00 EDT 2023

Salubrinal Enhances Cancer Cell Death during Glucose Deprivation through the Upregulation of xCT and Mitochondrial Oxidative Stress.

Biomedicines

Chen MC,Hsu LL,Wang SF,Pan YL,Lo JF,Yeh TS,Tseng LM,Lee HC

Published figure using EIF2S1 monoclonal antibody (Product # AHO0802) in Western Blot

Sat Aug 28 00:00:00 EDT 2021

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Nature

Guo X,Aviles G,Liu Y,Tian R,Unger BA,Lin YT,Wiita AP,Xu K,Correia MA,Kampmann M

Published figure using EIF2S1 monoclonal antibody (Product # AHO0802) in Western Blot

Sun Mar 01 00:00:00 EST 2020

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Nature

Guo X,Aviles G,Liu Y,Tian R,Unger BA,Lin YT,Wiita AP,Xu K,Correia MA,Kampmann M

Published figure using EIF2S1 monoclonal antibody (Product # AHO0802) in Western Blot

Sun Mar 01 00:00:00 EST 2020

Nucleocytoplasmic Proteomic Analysis Uncovers eRF1 and Nonsense-Mediated Decay as Modifiers of ALS/FTD C9orf72 Toxicity.

Neuron

Ortega JA,Daley EL,Kour S,Samani M,Tellez L,Smith HS,Hall EA,Esengul YT,Tsai YH,Gendron TF,Donnelly CJ,Siddique T,Savas JN,Pandey UB,Kiskinis E

AHO0802 was used in Western Blot to provide a resource for proteome-wide nucleocytoplasmic alterations across neurodegeneration-associated repeat expansion mutations and highlight eRF1 and NMD as therapeutic targets in C9orf72-associated ALS and/or FTD.

Wed Apr 08 00:00:00 EDT 2020

Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification.

Nature communications

Santos MCFD,Anderson CP,Neschen S,Zumbrennen-Bullough KB,Romney SJ,Kahle-Stephan M,Rathkolb B,Gailus-Durner V,Fuchs H,Wolf E,Rozman J,de Angelis MH,Cai WM,Rajan M,Hu J,Dedon PC,Leibold EA

AHO0802 was used in Western Blot to reveal a previously unidentified link between insulin processing and cellular iron deficiency that may have relevance to type 2 diabetes in humans.

Wed Jan 15 00:00:00 EST 2020

Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification.

Nature communications

Santos MCFD,Anderson CP,Neschen S,Zumbrennen-Bullough KB,Romney SJ,Kahle-Stephan M,Rathkolb B,Gailus-Durner V,Fuchs H,Wolf E,Rozman J,de Angelis MH,Cai WM,Rajan M,Hu J,Dedon PC,Leibold EA

AHO0802 was used in Western Blot to reveal a previously unidentified link between insulin processing and cellular iron deficiency that may have relevance to type 2 diabetes in humans.

Wed Jan 15 00:00:00 EST 2020

Fission Yeast Asc1 Stabilizes the Interaction between Eukaryotic Initiation Factor 3a and Rps0A/uS2 for Protein Synthesis.

Molecular and cellular biology

Wang YT,Chien YC,Hsiao WY,Wang CC,Wang SW

AHO0802 was used in Immunoprecipitation to find that Asc1 directly binds and stabilizes the interaction between small ribosomal protein Rps0A/uS2 and eukaryotic initiation factor 3a (eIF3a).

Tue Oct 01 00:00:00 EDT 2019

Inactivation of Ppp1r15a minimises weight gain and insulin resistance during caloric excess in female mice.

Scientific reports

Patel V,Bidault G,Chambers JE,Carobbio S,Everden AJT,Garcés C,Dalton LE,Gribble FM,Vidal-Puig A,Marciniak SJ

AHO0802 was used in Western Blotting to study the the role of the eIF2α phosphatases in regulating body weight.

Wed Feb 27 00:00:00 EST 2019

A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α.

MethodsX

Silva RC,Castilho BA,Sattlegger E

AHO0802 was used in Western Blotting to develop a rapid protein extraction method for mammalian cell cultures.

Thu Oct 01 00:00:00 EDT 2020

Extracellular and ER-stored Ca²⁺ contribute to BIRD-2-induced cell death in diffuse large B-cell lymphoma cells.

Cell death discovery

Bittremieux M,La Rovere RM,Schuermans M,Luyten T,Mikoshiba K,Vangheluwe P,Parys JB,Bultynck G

Published figure using eIF2a monoclonal antibody (Product # AHO0802) in Western Blot

Wed Sep 30 00:00:00 EDT 2020

Ebola Virus Does Not Induce Stress Granule Formation during Infection and Sequesters Stress Granule Proteins within Viral Inclusions.

Journal of virology

Nelson EV,Schmidt KM,Deflubé LR,Doğanay S,Banadyga L,Olejnik J,Hume AJ,Ryabchikova E,Ebihara H,Kedersha N,Ha T,Mühlberger E

Published figure using eIF2a monoclonal antibody (Product # AHO0802) in Western Blot

Mon Aug 15 00:00:00 EDT 2016

Infantile Cirrhosis, Growth Impairment, and Neurodevelopmental Anomalies Associated with Deficiency of PPP1R15B.

The Journal of pediatrics

Mohammad S,Wolfe LA,Stöbe P,Biskup S,Wainwright MS,Melin-Aldana H,Malladi P,Muenke M,Gahl WA,Whitington PF

AHO0802 was used in western blot to use whole-exome sequencing in a sibling pair with undetermined liver disease to identify mutations

Thu Dec 01 00:00:00 EST 2016

Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response.

Journal of cell science

Silva RC,Sattlegger E,Castilho BA

AHO0802 was used in western blot to propose that GCN2 is an important sensor of the state of the actin cytoskeleton

Thu Dec 15 00:00:00 EST 2016

Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation.

eLife

Chambers JE,Dalton LE,Clarke HJ,Malzer E,Dominicus CS,Patel V,Moorhead G,Ron D,Marciniak SJ

Published figure using eIF2a monoclonal antibody (Product # AHO0802) in Western Blot

Mon Mar 16 00:00:00 EDT 2015

Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation.

eLife

Chambers JE,Dalton LE,Clarke HJ,Malzer E,Dominicus CS,Patel V,Moorhead G,Ron D,Marciniak SJ

Published figure using eIF2a monoclonal antibody (Product # AHO0802) in Western Blot

Mon Mar 16 00:00:00 EDT 2015

Generation and characterization of an analog-sensitive PERK allele.

Cancer biology & therapy

Maas NL,Singh N,Diehl JA

AHO0802 was used in western blot to study PERK signaling using an analog-sensitive PERK allele that accepts N(6)-alkylated ATP analogs

Fri Aug 01 00:00:00 EDT 2014

Endoplasmic reticulum stress induced by aqueous extracts of cigarette smoke in 3T3 cells activates the unfolded-protein-response-dependent PERK pathway of cell survival.

Free radical biology & medicine

Hengstermann A,Müller T

AHO0802 was used in western blot to investigate how cigarette smoke induces endoplasmic reticulum stress

Sat Mar 15 00:00:00 EDT 2008

Enhanced antiglioma activity of chimeric HCMV/HSV-1 oncolytic viruses.

Gene therapy

Shah AC,Parker JN,Gillespie GY,Lakeman FD,Meleth S,Markert JM,Cassady KA

AHO0802 was used in western blot to characterize herpes simplex virus-1 gamma(1)34.5 mutants

Sun Jul 01 00:00:00 EDT 2007

Human cytomegalovirus TRS1 and IRS1 gene products block the double-stranded-RNA-activated host protein shutoff response induced by herpes simplex virus type 1 infection.

Journal of virology

Cassady KA

AHO0802 was used in western blot to elucidate how human cytomegalovirus evades PKR-mediated protein shutoff and maintains late viral protein synthesis

Fri Jul 01 00:00:00 EDT 2005

VSV replication in neurons is inhibited by type I IFN at multiple stages of infection.

Virology

Trottier MD,Palian BM,Reiss CS

Tue Mar 15 00:00:00 EST 2005

Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress.

The Journal of biological chemistry

Ramos-Castañeda J,Park YN,Liu M,Hauser K,Rudolph H,Shull GE,Jonkman MF,Mori K,Ikeda S,Ogawa H,Arvan P

Fri Mar 11 00:00:00 EST 2005

Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress.

The Journal of biological chemistry

Ramos-Castañeda J,Park YN,Liu M,Hauser K,Rudolph H,Shull GE,Jonkman MF,Mori K,Ikeda S,Ogawa H,Arvan P

Fri Mar 11 00:00:00 EST 2005

Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress.

The Journal of biological chemistry

Ramos-Castañeda J,Park YN,Liu M,Hauser K,Rudolph H,Shull GE,Jonkman MF,Mori K,Ikeda S,Ogawa H,Arvan P

Fri Mar 11 00:00:00 EST 2005

The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas.

Molecular and cellular biology

Zhang P,McGrath B,Li S,Frank A,Zambito F,Reinert J,Gannon M,Ma K,McNaughton K,Cavener DR

Sat Jun 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A

View more View less

Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1

View more View less

UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6

View more View less

Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665

View more View less

Function(s)

translation initiation factor activity protein binding ribosome binding poly(A) RNA binding nucleic acid binding RNA binding translation initiation factor

Process(es)

positive regulation of defense response to virus by host translational initiation aging negative regulation of translational initiation in response to stress cellular response to amino acid starvation cellular response to heat cellular response to UV response to endoplasmic reticulum stress PERK-mediated unfolded protein response protein autophosphorylation transmembrane transport mitophagy in response to mitochondrial depolarization xenophagy positive regulation of neuron death response to manganese-induced endoplasmic reticulum stress positive regulation of type B pancreatic cell apoptotic process translation regulation of translational initiation in response to stress regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

EIF2S1 Monoclonal Antibody (EIF2-alpha)

EIF2S1 Antibody (AHO0802) in ICC/IF

Product Details

AHO0802

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!