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FIGURE: 1 / 1
EPHA1 Antibody (CF809675) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHA1 (RC213689, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHA1. Positive lysates LY401608 (100 µg) and LC401608 (20 µg) can be purchased separately from OriGene.
Product Details
CF809675
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2D6
Immunogen
Human recombinant protein fragment corresponding to amino acids 24-327 of human EPHA1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embryonic stem cell kinase; EPH tyrosine kinase; EPH tyrosine kinase 1; ephrin receptor EphA1; Ephrin type-A receptor 1; erythropoietin-producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; hEpha1; mEpha1; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH; Tyrosine-protein kinase receptor ESK
Gene Aliases: 5730453L17Rik; AL033318; EPH; EPHA1; EPHT; EPHT1; Esk
UniProt ID: (Human) P21709, (Mouse) Q60750
Entrez Gene ID: (Human) 2041, (Mouse) 13835, (Rat) 312279
protein kinase activity
transmembrane-ephrin receptor activity
ATP binding
protein kinase binding
nucleotide binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
angiogenesis
positive regulation of cell-matrix adhesion
negative regulation of protein kinase activity
cell surface receptor signaling pathway
positive regulation of cell proliferation
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
negative regulation of cell migration
substrate adhesion-dependent cell spreading
somatic stem cell population maintenance
regulation of GTPase activity
positive regulation of angiogenesis
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of stress fiber assembly
activation of GTPase activity
protein phosphorylation
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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