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EPO Antibody (BS-2343R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product Details
BS-2343R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Erythropoietin, amino acids 101-193.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epoetin; Erythropoietin; MGC138142
Gene Aliases: EP; EPO; MVCD2
UniProt ID: (Human) P01588, (Mouse) P07321, (Rat) P29676
Entrez Gene ID: (Human) 2056, (Mouse) 13856, (Rat) 24335
negative regulation of transcription from RNA polymerase II promoter
apoptotic process
acute-phase response
signal transduction
embryo implantation
aging
blood circulation
positive regulation of cell proliferation
response to salt stress
negative regulation of calcium ion transport into cytosol
positive regulation of neuron projection development
peptidyl-serine phosphorylation
erythrocyte differentiation
activation of protein kinase activity
response to lipopolysaccharide
response to vitamin A
response to testosterone
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
hemoglobin biosynthetic process
erythrocyte maturation
response to estrogen
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of Ras protein signal transduction
response to axon injury
response to electrical stimulus
response to hyperoxia
regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of ERK1 and ERK2 cascade
response to interleukin-1
cellular hyperosmotic response
negative regulation of neuron death
negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
negative regulation of erythrocyte apoptotic process
negative regulation of cation channel activity
angiogenesis
response to hypoxia
heart morphogenesis
regulation of transcription from RNA polymerase II promoter
negative regulation of myeloid cell apoptotic process
negative regulation of apoptotic process
positive regulation of neuron differentiation
positive regulation of protein kinase activity
cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
visceral serous pericardium development
response to nutrient
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