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Product Details
CF804606
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1H6
Immunogen
Human recombinant protein fragment corresponding to amino acids 992-1308 of human ERBB4 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
Gene Aliases: ALS19; ERBB4; HER4; p180erbB4
UniProt ID: (Human) Q15303
Entrez Gene ID: (Human) 2066
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
protein binding
ATP binding
protein homodimerization activity
transcription regulatory region DNA binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
transmembrane signal receptor
MAPK cascade
neural crest cell migration
positive regulation of protein phosphorylation
transcription, DNA-templated
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
heart development
lactation
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
embryonic pattern specification
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
cell migration
peptidyl-tyrosine phosphorylation
central nervous system morphogenesis
olfactory bulb interneuron differentiation
regulation of cell migration
ERBB2 signaling pathway
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of GTPase activity
mitochondrial fragmentation involved in apoptotic process
cell fate commitment
positive regulation of transcription, DNA-templated
protein autophosphorylation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of cardiac muscle cell proliferation
mammary gland epithelial cell differentiation
mammary gland alveolus development
cardiac muscle tissue regeneration
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein localization to cell surface
regulation of cell motility
positive regulation of STAT protein import into nucleus
negative regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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