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ERCC1 Antibody (PA5-77971) in ICC/IF
Immunofluorescence analysis of ERCC1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ERCC1 Polyclonal Antibody (Product # PA5-77971) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a di... View More
Product Details
PA5-77971
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735768
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat2
Predicted Reactivity: Mouse (86%), Pig (87%), Chimpanzee (100%), Bovine (87%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. It is responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). It participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Gene Aliases: COFS4; ERCC1; RAD10; UV20
UniProt ID: (Human) P07992
Entrez Gene ID: (Human) 2067, (Rat) 292673
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
damaged DNA binding
single-stranded DNA binding
protein binding
protein C-terminus binding
protein domain specific binding
structure-specific DNA binding
molecular_function
TBP-class protein binding
endodeoxyribonuclease
pyrimidine dimer repair by nucleotide-excision repair
replicative cell aging
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
double-strand break repair
DNA recombination
mitotic recombination
syncytium formation
response to oxidative stress
spermatogenesis
response to nutrient
cell proliferation
male gonad development
UV protection
response to sucrose
response to X-ray
multicellular organism aging
negative regulation of telomere maintenance
nucleotide-excision repair, DNA incision
post-embryonic hemopoiesis
multicellular organism growth
interstrand cross-link repair
isotype switching
oogenesis
embryonic organ development
global genome nucleotide-excision repair
t-circle formation
positive regulation of t-circle formation
DNA catabolic process, endonucleolytic
cellular response to DNA damage stimulus
germ cell development
cell development
chromosome organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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