Proteintech

ERp57/ERp60 Polyclonal Antibody

ERp57/ERp60 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

ERp57/ERp60 Antibody (15967-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (Product # 15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ERp57/ERp60 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ERp57/ERp60 Antibody in Western Blot (WB)

ERp57/ERp60 Antibody in Flow Cytometry (Flow)

ERp57/ERp60 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15967-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ERp57/ERp60 Fusion Protein Ag8741 (161-505 aa encoded by BC014433)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DDSFSEAHS EFLKAASNLR DNYRFAHTNV ESLVNEYDDN GEGIILFRPS HLTNKFEDKT VAYTEQKMTS GKIKKFIQEN IFGICPHMTE DNKDLIQGKD LLIAYYDVDY EKNAKGSNYW RNRVMMVAKK FLDAGHKLNF AVASRKTFSH ELSDFGLEST AGEIPVVAIR TAKGEKFVMQ EEFSRDGKAL ERFLQDYFDG NLKRYLKSEP IPESNDGPVK VVVAENFDEI VNNENKDVLI EFYAPWCGHC KNLEPKYKEL GEKLSKDPNI VIAKMDATAN DVPSPYEVRG FPTIYFSPAN KKLNPKKYEG GRELSDFISY LQREATNPPV IQEEKPKKKK KAQEDL (161-505 aa encoded by BC014433)

Target Information

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nutrient deprivation alters the rate of COPII coat assembly to tune secretory protein transport.

Research square

Kasberg W,Luong P,Swift KA,Audhya A

Fri Mar 17 00:00:00 EDT 2023

Significance of Immunogenic Cell Death-Related Prognostic Gene Signature in Cervical Cancer Prognosis and Anti-Tumor Immunity.

Journal of inflammation research

Jiang S,Cui Z,Zheng J,Wu Q,Yu H,You Y,Zheng C,Sun Y

Wed May 31 00:00:00 EDT 2023

Significance of Immunogenic Cell Death-Related Prognostic Gene Signature in Cervical Cancer Prognosis and Anti-Tumor Immunity.

Journal of inflammation research

Jiang S,Cui Z,Zheng J,Wu Q,Yu H,You Y,Zheng C,Sun Y

Wed May 31 00:00:00 EDT 2023

Significance of Immunogenic Cell Death-Related Prognostic Gene Signature in Cervical Cancer Prognosis and Anti-Tumor Immunity.

Journal of inflammation research

Jiang S,Cui Z,Zheng J,Wu Q,Yu H,You Y,Zheng C,Sun Y

Wed May 31 00:00:00 EDT 2023

Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.

Redox biology

Ge X,He Z,Cao C,Xue T,Jing J,Ma R,Zhao W,Liu L,Jueraitetibaike K,Ma J,Feng Y,Qian Z,Zou Z,Chen L,Fu C,Song N,Yao B

Mon Aug 01 00:00:00 EDT 2022

MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

Genomics

Gu X,Li X,Zhang X,Feng R,Zheng M,Liu L,Yang J,Sun H,Zhang Q,Bian T,Zhao H,Liu Y

Fri Jul 01 00:00:00 EDT 2022

Protein Disulfide-Isomerase A3 Is a Robust Prognostic Biomarker for Cancers and Predicts the Immunotherapy Response Effectively.

Frontiers in immunology

Tu Z,Ouyang Q,Long X,Wu L,Li J,Zhu X,Huang K

Tue Apr 12 00:00:00 EDT 2022

Vitamin D₃ in ovarian antral follicles of mature gilts: Expression of its receptors and metabolic enzymes, concentration in follicular fluid and effect on steroid secretion in vitro.

Theriogenology

Grzesiak M,Knapczyk-Stwora K,Slomczynska M

Fri Jan 15 00:00:00 EST 2021

Vitamin D₃ in ovarian antral follicles of mature gilts: Expression of its receptors and metabolic enzymes, concentration in follicular fluid and effect on steroid secretion in vitro.

Theriogenology

Grzesiak M,Knapczyk-Stwora K,Slomczynska M

Fri Jan 15 00:00:00 EST 2021

Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway.

Frontiers in pharmacology

Sun J,Chen W,Li S,Yang S,Zhang Y,Hu X,Qiu H,Wu J,Xu S,Chu T

Sat Oct 16 00:00:00 EDT 2021

miRNA-mRNA Regulatory Network Reveals miRNAs in HCT116 in Response to Folic Acid Deficiency via Regulating Vital Genes of Endoplasmic Reticulum Stress Pathway.

BioMed research international

Lu L,Zhao H,Liu J,Zhang Y,Wang X

Mon May 31 00:00:00 EDT 2021

Calnexin mediates the maturation of GPI-anchors through ER retention.

The Journal of biological chemistry

Guo XY,Liu YS,Gao XD,Kinoshita T,Fujita M

Fri Nov 27 00:00:00 EST 2020

Identification of PGAM1 as a putative therapeutic target for pancreatic ductal adenocarcinoma metastasis using quantitative proteomics.

OncoTargets and therapy

Liu X,Weng Y,Liu P,Sui Z,Zhou L,Huang Y,Zhang L,Zhang Y,Tan X

Fri Mar 18 00:00:00 EDT 2022

Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.

Cell reports

Jones JR,Kong L,Hanna MG,Hoffman B,Krencik R,Bradley R,Hagemann T,Choi J,Doers M,Dubovis M,Sherafat MA,Bhattacharyya A,Kendziorski C,Audhya A,Messing A,Zhang SC

Tue Oct 23 00:00:00 EDT 2018

Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.

Cell reports

Jones JR,Kong L,Hanna MG,Hoffman B,Krencik R,Bradley R,Hagemann T,Choi J,Doers M,Dubovis M,Sherafat MA,Bhattacharyya A,Kendziorski C,Audhya A,Messing A,Zhang SC

Tue Oct 23 00:00:00 EDT 2018

The Circadian Gene Clock Regulates Bone Formation Via PDIA3.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Yuan G,Hua B,Yang Y,Xu L,Cai T,Sun N,Yan Z,Lu C,Qian R

Sat Apr 01 00:00:00 EDT 2017

Erdj3 Has an Essential Role for Z Variant Alpha-1-Antitrypsin Degradation.

Journal of cellular biochemistry

Khodayari N,Marek G,Lu Y,Krotova K,Wang RL,Brantly M

Sun Oct 01 00:00:00 EDT 2017

Erdj3 Has an Essential Role for Z Variant Alpha-1-Antitrypsin Degradation.

Journal of cellular biochemistry

Khodayari N,Marek G,Lu Y,Krotova K,Wang RL,Brantly M

Sun Oct 01 00:00:00 EDT 2017

Effect of Subcellular Translocation of Protein Disulfide Isomerase on Tetrachlorobenzoquinone-Induced Signaling Shift from Endoplasmic Reticulum Stress to Apoptosis.

Chemical research in toxicology

Liu Z,Wang Y,Wang Y,Dong W,Xia X,Song E,Song Y

Mon Oct 16 00:00:00 EDT 2017

Effect of Subcellular Translocation of Protein Disulfide Isomerase on Tetrachlorobenzoquinone-Induced Signaling Shift from Endoplasmic Reticulum Stress to Apoptosis.

Chemical research in toxicology

Liu Z,Wang Y,Wang Y,Dong W,Xia X,Song E,Song Y

Mon Oct 16 00:00:00 EDT 2017

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Oncotarget

Samanta S,Tamura S,Dubeau L,Mhawech-Fauceglia P,Miyagi Y,Kato H,Lieberman R,Buckanovich RJ,Lin YG,Neamati N

Tue Nov 28 00:00:00 EST 2017

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Oncotarget

Samanta S,Tamura S,Dubeau L,Mhawech-Fauceglia P,Miyagi Y,Kato H,Lieberman R,Buckanovich RJ,Lin YG,Neamati N

Tue Nov 28 00:00:00 EST 2017

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Oncotarget

Samanta S,Tamura S,Dubeau L,Mhawech-Fauceglia P,Miyagi Y,Kato H,Lieberman R,Buckanovich RJ,Lin YG,Neamati N

Tue Nov 28 00:00:00 EST 2017

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Oncotarget

Samanta S,Tamura S,Dubeau L,Mhawech-Fauceglia P,Miyagi Y,Kato H,Lieberman R,Buckanovich RJ,Lin YG,Neamati N

Tue Nov 28 00:00:00 EST 2017

Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer.

Oncotarget

Wang K,Li H,Chen R,Zhang Y,Sun XX,Huang W,Bian H,Chen ZN

Tue Nov 14 00:00:00 EST 2017

Clock mediates liver senescence by controlling ER stress.

Aging

Yuan G,Hua B,Cai T,Xu L,Li E,Huang Y,Sun N,Yan Z,Lu C,Qian R

Fri Dec 22 00:00:00 EST 2017

Clock mediates liver senescence by controlling ER stress.

Aging

Yuan G,Hua B,Cai T,Xu L,Li E,Huang Y,Sun N,Yan Z,Lu C,Qian R

Fri Dec 22 00:00:00 EST 2017

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.

The EMBO journal

Johnson A,Bhattacharya N,Hanna M,Pennington JG,Schuh AL,Wang L,Otegui MS,Stagg SM,Audhya A

Thu Mar 12 00:00:00 EDT 2015

TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.

The EMBO journal

Johnson A,Bhattacharya N,Hanna M,Pennington JG,Schuh AL,Wang L,Otegui MS,Stagg SM,Audhya A

Thu Mar 12 00:00:00 EDT 2015

MicroRNA directly enhances mitochondrial translation during muscle differentiation.

Cell

Zhang X,Zuo X,Yang B,Li Z,Xue Y,Zhou Y,Huang J,Zhao X,Zhou J,Yan Y,Zhang H,Guo P,Sun H,Guo L,Zhang Y,Fu XD

Thu Jul 31 00:00:00 EDT 2014

MicroRNA directly enhances mitochondrial translation during muscle differentiation.

Cell

Zhang X,Zuo X,Yang B,Li Z,Xue Y,Zhou Y,Huang J,Zhao X,Zhou J,Yan Y,Zhang H,Guo P,Sun H,Guo L,Zhang Y,Fu XD

Thu Jul 31 00:00:00 EDT 2014

Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.

Science China. Life sciences

Zhao Q,Feng Y,Jia X,Yin L,Zheng Y,Ouyang D,Zhou H,Zhang L

Sat Mar 01 00:00:00 EST 2014

Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.

Science China. Life sciences

Zhao Q,Feng Y,Jia X,Yin L,Zheng Y,Ouyang D,Zhou H,Zhang L

Sat Mar 01 00:00:00 EST 2014

Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.

Science China. Life sciences

Zhao Q,Feng Y,Jia X,Yin L,Zheng Y,Ouyang D,Zhou H,Zhang L

Sat Mar 01 00:00:00 EST 2014

Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.

Science China. Life sciences

Zhao Q,Feng Y,Jia X,Yin L,Zheng Y,Ouyang D,Zhou H,Zhang L

Sat Mar 01 00:00:00 EST 2014

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Molecular biology and evolution

Chen K,Huang C,Yuan J,Cheng H,Zhou R

Fri Aug 01 00:00:00 EDT 2014

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Molecular biology and evolution

Chen K,Huang C,Yuan J,Cheng H,Zhou R

Fri Aug 01 00:00:00 EDT 2014

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Cheng KC,Huang HH,Hung CT,Chen CC,Wu WC,Suen JL,Chen KJ,Wu YJ,Chang CH

Sun Dec 01 00:00:00 EST 2013

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Cheng KC,Huang HH,Hung CT,Chen CC,Wu WC,Suen JL,Chen KJ,Wu YJ,Chang CH

Sun Dec 01 00:00:00 EST 2013

Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.

The Journal of clinical endocrinology and metabolism

Liu XM,Ding GL,Jiang Y,Pan HJ,Zhang D,Wang TT,Zhang RJ,Shu J,Sheng JZ,Huang HF

Mon Oct 01 00:00:00 EDT 2012

Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.

The Journal of clinical endocrinology and metabolism

Liu XM,Ding GL,Jiang Y,Pan HJ,Zhang D,Wang TT,Zhang RJ,Shu J,Sheng JZ,Huang HF

Mon Oct 01 00:00:00 EDT 2012

Proteomics changes in adhesion molecules: a driving force for vascular smooth muscle cell phenotypic switch.

Molecular bioSystems

Rocchiccioli S,Ucciferri N,Comelli L,Trivella MG,Citti L,Cecchettini A

Sun Apr 01 00:00:00 EDT 2012

Proteomics changes in adhesion molecules: a driving force for vascular smooth muscle cell phenotypic switch.

Molecular bioSystems

Rocchiccioli S,Ucciferri N,Comelli L,Trivella MG,Citti L,Cecchettini A

Sun Apr 01 00:00:00 EDT 2012

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Marine drugs

Su CC,Su JH,Lin JJ,Chen CC,Hwang WI,Huang HH,Wu YJ

Thu Dec 01 00:00:00 EST 2011

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Marine drugs

Su CC,Su JH,Lin JJ,Chen CC,Hwang WI,Huang HH,Wu YJ

Thu Dec 01 00:00:00 EST 2011

Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells.

Marine drugs

Liu CI,Chen CC,Chen JC,Su JH,Huang HH,Chen JY,Wu YJ

Fri Feb 10 00:00:00 EST 2012

Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells.

Marine drugs

Liu CI,Chen CC,Chen JC,Su JH,Huang HH,Chen JY,Wu YJ

Fri Feb 10 00:00:00 EST 2012

Bioinformatics

Protein Aliases: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum protein; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ER-60 protease; ERp 57; ERp60; glucose regulated protein, 58 kDa; glucose regulated protein, 58kDa; GRP 57; GRP 58; HIP-70; oxidoreductase ERp57; p58; PDIA 3; phospholipase C, alpha; phospholipase C-alpha; PI PLC; protein disulfide isomerase associated 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3; Q-2

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Gene Aliases: 58kDa; ER60; Erp; ERP57; ERP60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PDI; PDI-Q2; PDIA3; PI-PLC; PLC[a]; Plca

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UniProt ID: (Human) P30101, (Mouse) P27773, (Rat) P11598

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Entrez Gene ID: (Human) 2923, (Mouse) 14827, (Rat) 29468

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Function(s)

protein disulfide isomerase activity cysteine-type endopeptidase activity phospholipase C activity protein binding disulfide oxidoreductase activity poly(A) RNA binding peptidase activity isomerase activity protein-disulfide reductase (glutathione) activity MHC class I protein binding chaperone

Process(es)

antigen processing and presentation of peptide antigen via MHC class I protein folding proteolysis protein import into nucleus protein retention in ER lumen signal transduction protein folding in endoplasmic reticulum response to endoplasmic reticulum stress cell redox homeostasis oxidation-reduction process positive regulation of extrinsic apoptotic signaling pathway cellular response to vitamin D positive regulation of protein folding

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ERp57/ERp60 Polyclonal Antibody

ERp57/ERp60 Antibody (15967-1-AP) in ICC/IF

Product Details

15967-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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